Down-Regulation of miR-129-5p Inhibits Growth and Induces Apoptosis in Laryngeal Squamous Cell Carcinoma by Targeting APC

被引：63
|
作者：
Li, MingHua [1 ]
Tian, LinLi [2 ]
Wang, Lin [1 ]
Yao, HongChao [1 ]
Zhang, JiaRui [2 ]
Lu, JianGuang [2 ]
Sun, YaNan [1 ]
Gao, Xu [3 ]
Xiao, Hui [2 ]
Liu, Ming [1 ]
机构：
[1] Harbin Med Univ, Affiliated Hosp 2, Dept Otolaryngol Head & Neck Surg, Serv Head & Neck Surg, Harbin, Peoples R China
[2] Harbin Med Univ, Affiliated Hosp 2, Dept Otolaryngol Head & Neck Surg, Serv Laryngol, Harbin, Peoples R China
[3] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin, Peoples R China
来源：
PLOS ONE | 2013年 / 8卷 / 10期
基金：
高等学校博士学科点专项科研基金; 美国国家科学基金会;
关键词：
WNT SIGNALING PATHWAY; BETA-CATENIN; MICRORNA EXPRESSION; CANCER; PROLIFERATION; TUMORIGENESIS; INVASION; MIRNAS; ROLES; LEADS;
D O I：
10.1371/journal.pone.0077829
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
miRNAs regulate gene expression and are key mediators of tumourigenesis. miR-129 has diverse effects in tumours, but its role in laryngeal squamous cell carcinoma (LSCC) remains unknown. This article focuses on the role of miR-129-5p in LSCC. We show miR-129-5p is upregulated in primary LSCC tumours and correlated with advanced disease. Down-regulating miR-129-5p suppressed cell proliferation and migration, and caused cell cycle arrest in Hep-2 cell lines. Downregulation of miR-129-5p alone is sufficient to induce apoptosis both in vivo and in vitro. Moreover, the growth of LSCC xenograft exposed to miR-129-5p antisense oligonucleotides (ASO) in BALB/c mice was markedly inhibited. In addition, we found that miR-129-5p targeted adenomatous polyposis coli (APC) to release inhibition of Wnt signalling causing cell growth and tumourigenesis. Our results suggest miR-129-5p functions as an oncogene in LSCC by repressing APC and is a potential therapeutic target for LSCC.
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页数：10
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