Down-Regulation of miR-129-5p Inhibits Growth and Induces Apoptosis in Laryngeal Squamous Cell Carcinoma by Targeting APC

被引：65

作者：

Li, MingHua ^{[1
]}

Tian, LinLi ^{[2
]}

Wang, Lin ^{[1
]}

Yao, HongChao ^{[1
]}

Zhang, JiaRui ^{[2
]}

Lu, JianGuang ^{[2
]}

Sun, YaNan ^{[1
]}

Gao, Xu ^{[3
]}

Xiao, Hui ^{[2
]}

Liu, Ming ^{[1
]}

机构：

[1] Harbin Med Univ, Affiliated Hosp 2, Dept Otolaryngol Head & Neck Surg, Serv Head & Neck Surg, Harbin, Peoples R China

[2] Harbin Med Univ, Affiliated Hosp 2, Dept Otolaryngol Head & Neck Surg, Serv Laryngol, Harbin, Peoples R China

[3] Harbin Med Univ, Dept Biochem & Mol Biol, Harbin, Peoples R China

来源：

PLOS ONE | 2013年 / 8卷 / 10期

基金：

高等学校博士学科点专项科研基金; 美国国家科学基金会;

关键词：

WNT SIGNALING PATHWAY; BETA-CATENIN; MICRORNA EXPRESSION; CANCER; PROLIFERATION; TUMORIGENESIS; INVASION; MIRNAS; ROLES; LEADS;

D O I：

10.1371/journal.pone.0077829

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

miRNAs regulate gene expression and are key mediators of tumourigenesis. miR-129 has diverse effects in tumours, but its role in laryngeal squamous cell carcinoma (LSCC) remains unknown. This article focuses on the role of miR-129-5p in LSCC. We show miR-129-5p is upregulated in primary LSCC tumours and correlated with advanced disease. Down-regulating miR-129-5p suppressed cell proliferation and migration, and caused cell cycle arrest in Hep-2 cell lines. Downregulation of miR-129-5p alone is sufficient to induce apoptosis both in vivo and in vitro. Moreover, the growth of LSCC xenograft exposed to miR-129-5p antisense oligonucleotides (ASO) in BALB/c mice was markedly inhibited. In addition, we found that miR-129-5p targeted adenomatous polyposis coli (APC) to release inhibition of Wnt signalling causing cell growth and tumourigenesis. Our results suggest miR-129-5p functions as an oncogene in LSCC by repressing APC and is a potential therapeutic target for LSCC.

引用

页数：10

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