Differential expression of two winter wheat alpha-tubulin genes during cold acclimation

被引：14
作者：
Christov, Nikolai K. [1 ]
Imai, Ryozo [2 ]
Blume, Yaroslav [3 ]
机构：
[1] AgroBioInstitute, Sofia 1164, Bulgaria
[2] Natl Agr Res Ctr Hokkaido Reg, Crop Cold Tolerance Res Team, Toyohira Ku, Sapporo, Hokkaido 0628555, Japan
[3] Natl Acad Sci Ukraine, Inst Cell Biol & Genet Engn, UA-03680 Kiev, Ukraine
来源：
CELL BIOLOGY INTERNATIONAL | 2008年 / 32卷 / 05期
关键词：
Alpha-tubulin; WCS120; Cold acclimation; Wheat; Triticum aestivum; Abiotic stress;
D O I：
10.1016/j.cellbi.2007.11.014
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Overwintering crops, such as winter wheat, display significant increase in freezing tolerance during a period of cold acclimation (CA). To gain better understanding of molecular mechanisms of CA, it is important to unravel functions and regulations of CA-associated genes. Differential screening of a cDNA library constructed from cold-acclimated crown tissue of winter wheat identified an alpha-tubulin cDNA clone named wca18g11 that showed elevated expression upon cold acclimation. Nucleotide sequence analysis showed that the clone encoded a group 3 alpha-tubulin. Reverse transcription real-time PCR analysis of the expression of both wca18g11 clone and its closest paralogs of the wheat tubulin A-2 homeologous group during the course of cold acclimation revealed that both genes were differentially regulated with distinct expression patterns. The involvement of the two alpha-tubulin genes in cold acclimation and signal transduction is discussed. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：574 / 578
页数：5
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