Molecular mechanism of cell-autonomous circadian gene expression of Period2, a crucial regulator of the mammalian circadian clock

被引:61
作者
Akashi, M
Ichise, T
Mamine, T
Takumi, T [1 ]
机构
[1] Osaka Biosci Inst, Osaka 5650874, Japan
[2] Sony Corp, Mat Labs, Life Sci Lab, Shinagawa Ku, Tokyo 1440001, Japan
关键词
D O I
10.1091/mbc.E05-05-0396
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Although circadian transcription of Period2 (Per2) is fundamental for the generation of circadian rhythm, the molecular mechanism remains unclear. Here we report that cell-autonomous circadian transcription of Per2 is driven by two transcriptional elements, one for rhythm generation and the other for phase control. The former contains the E-box-like sequence (CACGTT) that is sufficient and indispensable to drive oscillation, and indeed circadian transcription factors site-specifically bind to it. Furthermore, the nature of this atypical E-box is different from that of the classical circadian E-box. The current feedback loop model is based mainly on Period1. Our results provide not only compelling evidence in support of this model but also an explanation for a general basic mechanism to produce various patterns in the phase and amplitude of cell-autonomous circadian gene expression.
引用
收藏
页码:555 / 565
页数:11
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