Cryo-electron tomography of bacterial viruses

被引：11

作者：

Guerrero-Ferreira, Ricardo C. ^{[1
]}

Wright, Elizabeth R. ^{[1
]}

机构：

[1] Emory Univ, Sch Med, Childrens Healthcare Atlanta, Div Pediat Infect Dis, Atlanta, GA 30322 USA

来源：

VIROLOGY | 2013年 / 435卷 / 01期

关键词：

Bacteriophage; Cryo-electron microscopy; Cryo-EM; Cryo-electron tomography; Cryo-ET; Sub-tomogram averaging; ELECTRON-MICROSCOPY; CAULOBACTER-CRESCENTUS; 3-DIMENSIONAL RECONSTRUCTION; MOLECULAR LANDSCAPES; BACTERIOPHAGE PRD1; STRUCTURAL-CHANGES; LIGHT-MICROSCOPY; TISSUE SECTIONS; HEAVY METALS; CELLS;

D O I：

10.1016/j.virol.2012.08.022

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacteriophage particles contain both simple and complex macromolecular assemblages and machines that enable them to regulate the infection process under diverse environmental conditions with a broad range of bacterial hosts. Recent developments in cryo-electron tomography (cryo-ET) make it possible to observe the interactions of bacteriophages with their host cells under native-state conditions at unprecedented resolution and in three-dimensions. This review describes the application of cryo-ET to studies of bacteriophage attachment, genome ejection, assembly and egress. Current topics of investigation and future directions in the field are also discussed. (C) 2012 Elsevier Inc. All rights reserved.

引用

页码：179 / 186

页数：8

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