IL-8 Release from Human Neutrophils Cultured with Pro-Haptenic Chemical Sensitizers

被引：4
|
作者：
Kiorpelidou, Eva [1 ]
Foster, Brian [1 ]
Farrell, John [1 ]
Ogese, Monday O. [1 ]
Faulkner, Lee [1 ]
Goldring, Chris E. [1 ]
Park, B. Kevin [1 ]
Naisbitt, Dean J. [1 ]
机构：
[1] Univ Liverpool, MRC Ctr Drug Safety Sci, Dept Pharmacol, Liverpool L69 3GE, Merseyside, England
来源：
CHEMICAL RESEARCH IN TOXICOLOGY | 2012年 / 25卷 / 10期
基金：
英国医学研究理事会;
关键词：
SKIN SENSITIZATION; PEPTIDE REACTIVITY; EUGENOL;
D O I：
10.1021/tx300350s
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Cytokine release from dendritic cells in vitro is a useful marker to discriminate between sensitizing and irritant haptenic chemicals. Unfortunately, pro-haptens, which gain reactivity following metabolic/auto activation, yield negative results. To overcome this, we exposed human neutrophils and THP-1 cells to haptens/pro-haptens and measured IL-8 release. Haptenic compounds stimulated IL-8 release in neutrophils and THP-1 cells. In contrast, the pro-haptens eugenol, isoeugenol, and 2-aminophenol stimulated high levels of IL-8 release from neutrophils alone. Neutrophil cytoldne release was reduced when glutathione was added. Cyp1A1/1B1/3A4 were not detectable in THP-1 cells or neutrophils; however, neutrophils expressed high levels of myeloperoxidase.
引用
收藏
页码：2054 / 2056
页数：3
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