IL-8 Release from Human Neutrophils Cultured with Pro-Haptenic Chemical Sensitizers

被引：4

作者：

Kiorpelidou, Eva ^{[1
]}

Foster, Brian ^{[1
]}

Farrell, John ^{[1
]}

Ogese, Monday O. ^{[1
]}

Faulkner, Lee ^{[1
]}

Goldring, Chris E. ^{[1
]}

Park, B. Kevin ^{[1
]}

Naisbitt, Dean J. ^{[1
]}

机构：

[1] Univ Liverpool, MRC Ctr Drug Safety Sci, Dept Pharmacol, Liverpool L69 3GE, Merseyside, England

来源：

CHEMICAL RESEARCH IN TOXICOLOGY | 2012年 / 25卷 / 10期

基金：

英国医学研究理事会;

关键词：

SKIN SENSITIZATION; PEPTIDE REACTIVITY; EUGENOL;

D O I：

10.1021/tx300350s

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

Cytokine release from dendritic cells in vitro is a useful marker to discriminate between sensitizing and irritant haptenic chemicals. Unfortunately, pro-haptens, which gain reactivity following metabolic/auto activation, yield negative results. To overcome this, we exposed human neutrophils and THP-1 cells to haptens/pro-haptens and measured IL-8 release. Haptenic compounds stimulated IL-8 release in neutrophils and THP-1 cells. In contrast, the pro-haptens eugenol, isoeugenol, and 2-aminophenol stimulated high levels of IL-8 release from neutrophils alone. Neutrophil cytoldne release was reduced when glutathione was added. Cyp1A1/1B1/3A4 were not detectable in THP-1 cells or neutrophils; however, neutrophils expressed high levels of myeloperoxidase.

引用

页码：2054 / 2056

页数：3

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