Hydrophobic interactions are the prevalent force in bromelain:Fab′ complex

被引：6
|
作者：
Gupta, P
Saleemuddin, M
Khan, RH [1 ]
机构：
[1] Aligarh Muslim Univ, Fac Life Sci, Interdisciplinary Biotechnol Unit, Aligarh 202002, Uttar Pradesh, India
[2] Aligarh Muslim Univ, Fac Life Sci, Dept Biochem, Aligarh 202002, Uttar Pradesh, India
来源：
BIOCHEMISTRY-MOSCOW | 2006年 / 71卷 / Suppl 1期
关键词：
bromelain; Fab '; immunocomplexes; hydrophobic interaction; electrostatic interaction; methanol; NaCl;
D O I：
10.1134/S0006297906130050
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Antibromelain polyclonal antibodies against stem bromelain were raised in male albino rabbits and the Fab' monomers isolated from the IgG of the immune sera as reported in our earlier communication (Gupta, P., Khan, R. H., and Saleemuddin, M. (2003) Biochim. Biophys. Acta, 1646, 131-135). Further, as evident from that communication, bromelain:Fab' complex has 1 : 1 stoichiometry. The stability of bromelain:Fab' complex (1 : 1) was investigated by far and near-UV CD and fluorescence measurements. Addition of up to 1.8 M NaCl caused no significant changes in fluorescence signals and near-UV CD peak pattern. However, the spectral studies together with gel filtration studies suggest dissociation of the complex beyond 5% (v/v) methanol. These results show that hydrophobic interactions play a pronounced role in the binding of Fab' to bromelain while electrostatic interactions may be less crucial.
引用
收藏
页码：S31 / S37
页数：7
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