Cell Wall Polysaccharide-Mediated Cadmium Tolerance Between Two Arabidopsis thaliana Ecotypes

被引:57
作者
Xiao, Yan [1 ,2 ]
Wu, Xiuwen [1 ,2 ]
Liu, Dong [1 ,2 ]
Yao, Junyue [1 ,2 ]
Liang, Guihong [1 ,2 ]
Song, Haixing [1 ,2 ]
Ismail, Abdelbagi M. [3 ]
Luo, Jin-Song [1 ,2 ]
Zhang, Zhenhua [1 ,2 ]
机构
[1] Hunan Agr Univ, Coll Resources & Environm Sci, Southern Reg Collaborat Innovat Ctr Grain & Oil C, Changsha, Peoples R China
[2] Natl Ctr Oilseed Crops Improvement, Hunan Branch, Changsha, Peoples R China
[3] Int Rice Res Inst, Manila, Philippines
来源
FRONTIERS IN PLANT SCIENCE | 2020年 / 11卷
基金
中国博士后科学基金; 国家重点研发计划; 中国国家自然科学基金;
关键词
cadmium tolerance; Arabidopsis thaliana ecotypes; phytoremediation; cell wall polysaccharides; pectin; cellulose; hemicellulose; XYLOGLUCAN ENDOTRANSGLUCOSYLASE/HYDROLASE GENE; EXPANSIN GENE; RISK-ASSESSMENT; EXPRESSION; ACCUMULATION; PROLINE; MECHANISMS; RESPONSES; TOXICITY; CAPACITY;
D O I
10.3389/fpls.2020.00473
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Cadmium (Cd) is a toxic metal element and the mechanism(s) underlying Cd tolerance in plants are still unclear. Increasingly more studies have been conducted on Cd binding to plant cell walls (CW) but most of them have focused on Cd fixation by CW pectin, and few studies have examined Cd binding to cellulose and hemicellulose. Here we found that Cd binding to CW pectin, cellulose, and hemicellulose was significantly higher in Tor-1, a Cd tolerant A. thaliana ecotype, than in Ph2-23, a sensitive ecotype, as were the concentrations of pectin, cellulose, and hemicellulose. Transcriptome analysis revealed that the genes regulating CW pectin, cellulose, and hemicellulose polysaccharide concentrations in Tor-1 differed significantly from those in Ph2-23. The expressions of most genes such as pectin methyl esterase inhibitors (PMEIs), pectin lyases, xyloglucan endotransglucosylase/hydrolase, expansins (EXPAs), and cellulose hydrolase were higher in Ph2-23, while the expressions of cellulose synthase-like glycosyltransferase 3 (CSLG3) and pectin ethyl esterase 4 (PAE4) were higher in Tor-1. The candidate genes identified here seem to regulate CW Cd fixation by polysaccharides. In conclusion, an increase in pectin demethylation activity, the higher concentration of cellulose and hemicellulose, regulated by related genes, in Tor-1 than in Ph2-23 are likely involved in enhanced Cd CW retention and reduce Cd toxicity.
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页数:13
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