Evaluation of separation quality in two-dimensional hyphenated chromatography

被引：23

作者：

Gong, F

Liang, YZ ^{[1
]}

Xu, QS

Chau, FT

Ng, KM

机构：

[1] Cent S Univ, Coll Chem & Chem Engn, Changsha 410083, Hunan, Peoples R China

[2] Hunan Univ, Dept Math Appl, Changsha 410082, Peoples R China

[3] Hong Kong Polytech Univ, Dept Appl Biol & Chem Technol, Kowloon, Hong Kong, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 2001年 / 450卷 / 1-2期

关键词：

hyphenated chromatography; separation quality; overlap index; D-optimum;

D O I：

10.1016/S0003-2670(01)01368-X

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this paper, a novel criterion named the overlap index (OVI) has been proposed to evaluate the separation quality in two-dimensional hyphenated chromatography. This criterion, which is obtained from the maximum value of the Gram determinant constructed by the key spectra, can reveal the degree of two-dimensional hyphenated chromatographic separation. The key spectra are selected based on the so-called D-optimal criterion. According to its geometrical sense meaning the volume spanned by the constructed vectors, OVI criterion can practically reflect the overlap degree of chromatographic peaks. The OVI criterion is further interpreted by comparing the resolution of two-dimensional data into pure chromatograms and spectra. The successful application of this OVI criterion to both of simulated two-dimensional hyphenated data and the real case in analyzing the six reference phenolic compounds from Ginkgo biloba L-leaves shows its reliability and practicality. (C) 2001 Elsevier Science B.V All rights reserved.

引用

页码：99 / 114

页数：16