Quorum sensing signal autoinducer-2 promotes root colonization ofBacillus velezensisSQR9 by affecting biofilm formation and motility

被引：41
作者：
Xiong, Qin [1 ]
Liu, Di [1 ]
Zhang, Huihui [2 ,3 ]
Dong, Xiaoyan [1 ]
Zhang, Guishan [1 ]
Liu, Yunpeng [1 ]
Zhang, Ruifu [1 ,2 ,3 ]
机构：
[1] Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Minist Agr, Key Lab Microbial Resources Collect & Preservat, Beijing 100081, Peoples R China
[2] Nanjing Agr Univ, Jiangsu Key Lab, Natl Engn Res Ctr Organ Based Fertilizers, Nanjing 210095, Peoples R China
[3] Nanjing Agr Univ, Engn Ctr Solid Organ Waste Utilizat, Natl Engn Res Ctr Organ Based Fertilizers, Nanjing 210095, Peoples R China
来源：
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2020年 / 104卷 / 16期
基金：
国家重点研发计划; 中国国家自然科学基金;
关键词：
AI-2; Bacillus velezensis; Biofilm formation; Motility; Quorum sensing; Root colonization; BACILLUS-SUBTILIS; ESCHERICHIA-COLI; LUXS; SYSTEM; AMYLOLIQUEFACIENS; BIOSYNTHESIS; GENES; ACID; COMMUNICATION; EXPRESSION;
D O I：
10.1007/s00253-020-10713-w
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Root colonization of beneficial rhizobacteria is critical for their beneficial effects. Quorum sensing (QS) has been reported to affect the colonization of many plant pathogens. However, how QS signals regulate root colonization of beneficial rhizobacteria is unclear. In this study, the QS signal AI-2 synthetase-encoding geneluxSwas completely deleted from the genome of the plant beneficial rhizobacteriumBacillus velezensisSQR9, and bioluminescence experiments showed that AI-2 production was blocked. Deletion ofluxSreduced biofilm formation, motility, and root colonization ofB. velezensisSQR9, while addition of exogenous AI-2 to the mutant restored this phenomenon. These results indicated that AI-2 positively affects the root colonization ofB. velezensisSQR9. This study provided new insights for enhancing the colonization of beneficial rhizobacteria.
引用
收藏
页码：7177 / 7185
页数：9
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