Effects of storage time and temperature on coagulation tests and factors in fresh plasma

被引：38
作者：
Feng, Limin [1 ]
Zhao, Ying [1 ]
Zhao, Hongcan [2 ]
Shao, Zhexin [3 ]
机构：
[1] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Lab Med, Hangzhou 310003, Zhejiang, Peoples R China
[2] First Peoples Hosp Hangzhou, Dept Lab Med, Hangzhou 310003, Peoples R China
[3] Zhejiang Univ, Coll Med, Affiliated Hosp 1, Dept Hosp Off, Hangzhou 310003, Zhejiang, Peoples R China
来源：
SCIENTIFIC REPORTS | 2014年 / 4卷
关键词：
PARTIAL THROMBOPLASTIN TIME; WHOLE-BLOOD; FACTOR-VIII; PROTHROMBIN TIME; FROZEN PLASMA; STABILITY; VARIABLES; EFFICACY; ASSAYS;
D O I：
10.1038/srep03868
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Coagulation tests and factors measurements have been widely applied in clinical practice. Pre-analytical conditions are very important in laboratory assessment. Here, we aim to determine the effects of storage time and temperature on activated partial thromboplastin time (APTT), fibrinogen (Fbg), prothrombin time (PT), the international normalized ratio (INR), thrombin time (TT), factor VIII activity (FVIII:C), and factor IX activity (FIX:C) in fresh plasma. Seventy-two blood samples were tested after storage for 0 (baseline), 2, 4, 6, 8, 12, and 24 h at 25 degrees C (room temperature) and 4 degrees C (refrigeration) in two centers. The mean percentage change of greater than 10% and the numbers of samples with greater than 10% percentage changes more than 25% were used to determine clinically relevant difference. We demonstrated that samples for Fbg, PT/INR, and TT could be safely stored for <= 24 h; FVIII:C for <= 2 h; FIX:C for <= 4 h both at 4 degrees C and 25 degrees C; and APTT for <= 12 h at 4 degrees C and <= 8 h at 25 degrees C.
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页数：5
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