Monoclonal antibody-quantum dots CdTe conjugate-based fluoroimmunoassay for the determination of aflatoxin B1 in peanuts

被引：68

作者：

Zhang, Zhaowei ^{[1
,2
,4
]}

Li, Yuanyuan ^{[1
,2
]}

Li, Peiwu ^{[1
,2
,4
,5
]}

Zhang, Qi ^{[1
,3
]}

Zhang, Wen ^{[1
,5
]}

Hu, Xiaofeng ^{[1
,2
]}

Ding, Xiaoxia ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Oil Crops Res Inst, Wuhan 430062, Peoples R China

[2] Minist Agr, Key Lab Detect Mycotoxins, Wuhan 430062, Peoples R China

[3] Minist Agr, Key Lab Biol & Genet Improvement Oil Crops, Wuhan 430062, Peoples R China

[4] Minist Agr, Lab Risk Assessment Oilseeds Prod Wuhan, Wuhan 430062, Peoples R China

[5] Minist Agr, Qual Inspect & Test Ctr Oilseeds Prod, Wuhan 430062, Peoples R China

来源：

FOOD CHEMISTRY | 2014年 / 146卷

基金：

中国国家自然科学基金;

关键词：

Quantum dots CdTe; Fluoroimmunoassay; Aflatoxin B-1; Peanuts; FOOD; CHROMATOGRAPHY; ELISA; CDSE; SCFV;

D O I：

10.1016/j.foodchem.2013.09.048

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

A fluoroimmunoassay towards aflatoxin B-1 (AFB(1)) was presented using quantum dots as the fluorescent label. The CdTe QDs were successfully linked to the monoclonal antibody against AFB(1). Based on the conjugated complexes, a novel direct competitive fluorescence-linked immunosorbent assay (cFLISA) was developed for AFB(1) detection. The 50% inhibition value (IC50) of the cFLISA was 0.149 ng/mL in peanuts matrix. The method performance included the limit of detection (LOD) of 0.016 ng/mL and considerable recoveries of 85-117% at three fortification levels (0.075, 0.15, and 0.3 ng/g) from spiked AFB(1) blank peanuts samples, along with coefficients of variation (CVs) below 10%. The cFLISA provided an alternative of rapid and sensitive detection for AFB(1) and, moreover provided great potential for multiplexed mycotoxins determination simultaneously. (C) 2013 Elsevier Ltd. All rights reserved.

引用

页码：314 / 319

页数：6

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