UV-absorption studies of interaction of karanjin and karanjachromene with ds. DNA: Evaluation of binding and antioxidant activity

被引：16

作者：

Arshad, Nasima ^{[1
]}

Rashid, Naghmana ^{[1
]}

Absar, Sajida ^{[1
]}

Abbasi, Muhammad S. A. ^{[1
]}

Saleem, Samreen ^{[2
]}

Mirza, Bushra ^{[2
]}

机构：

[1] Allama Iqbal Open Univ, Dept Chem, Islamabad 44000, Pakistan

[2] Quaid I Azam Univ, Dept Biochem, Islamabad 45320, Pakistan

来源：

CENTRAL EUROPEAN JOURNAL OF CHEMISTRY | 2013年 / 11卷 / 12期

关键词：

Flavonoids; UV-Vis spectroscopy; Interaction with chicken blood ds.DNA; Binding constants; Antioxidant activity; SCAVENGING ACTIVITY; FLAVONOIDS; COMPONENTS; MECHANISM; OXIDATION; DPPH;

D O I：

10.2478/s11532-013-0327-z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Two flavonoids, karanjin (Kj) and karanjachromene (Kc) have been investigated spectrophotometrically for their mode of interactions with double stranded (ds)-DNA at blood (7.4) and stomach (4.7) pH and at human body temperature (37A degrees C). Benesi-Hildebrand equation was used to evaluate the binding constants, K (b) . Binding constants at both pH values and at body temperature showed stronger binding of both the flavonoids and formation of 1:1 flavonoid-DNA complex via intercalative mode. However, K (b) values for karanjin were evaluated to be comparatively greater than karanjachromene at both pH values. The highest value of binding constant (1.32x10(5) M-1) for karanjin at blood pH (7.4) demonstrated its comparatively stronger binding and greater effectiveness at this pH. Standard Gibbs free energy changes (Delta G) of flavonoid-DNA complexes were calculated as negative values and indicative of spontaneity of their binding. Both flavonoids showed significant DNA protection activity.

引用

页码：2040 / 2047

页数：8

共 44 条

[1] Synthesis of novel benzofuran isoxazolines as protein tyrosine phosphatase 1B inhibitors [J].