3D Droplet Microfluidic Systems for High-Throughput Biological Experimentation

被引：50

作者：

Kang, Dong-Ku ^{[1
]}

Gong, Xiuqing ^{[1
]}

Cho, Soongwon ^{[1
]}

Kim, Jin-young ^{[1
]}

Edel, Joshua B. ^{[1
]}

Chang, Soo-Ik ^{[2
]}

Choo, Jaebum ^{[3
]}

deMello, Andrew J. ^{[1
]}

机构：

[1] Univ London Imperial Coll Sci Technol & Med, Dept Chem, London SW7 2AZ, England

[2] Chungbuk Natl Univ, Dept Biochem, Cheongjoo 361763, South Korea

[3] Hanyang Univ, Dept Bionano Technol, Ansan 426791, South Korea

来源：

ANALYTICAL CHEMISTRY | 2015年 / 87卷 / 21期

基金：

英国生物技术与生命科学研究理事会; 新加坡国家研究基金会; 英国工程与自然科学研究理事会;

关键词：

PROTEIN-PROTEIN INTERACTIONS; SINGLE-CELL ANALYSIS; PICOLITER-VOLUME; HODGKINS-DISEASE; CANCER; APOPTOSIS; CASPASE-3; GENERATION; GRADIENTS; MOLECULE;

D O I：

10.1021/acs.analchem.5b02402

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Herein, we describe the development of a multilayer droplet microfluidic system for creating concentration gradients and generating micro droplets of varying composition for high-throughput biochemical and cell-based screening applications. The 3D droplet-based microfluidic device consists of multiple PDMS layers, which are used to generate logarithmic concentration gradient reagent profiles. Parallel flow focusing structures are used to form picoliter-sized droplets of defined volumes but of varying composition. As proof of concept, we demonstrate rapid enzymatic activity assays and drug cytotoxicity assays on bacteria. The 3D droplet-based microfluidic platform has the potential to allow for high-efficiency and high-throughput analysis, overcoming the structural limitations of single layer microfluidic systems.

引用

页码：10770 / 10778

页数：9

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