An efficient fluorimetric method to measure the viability of intraerythrocytic Plasmodium falciparum

被引：12

作者：

Evers, Astrid ^{[1
]}

Heppner, Saskia ^{[1
]}

Leippe, Matthias ^{[1
]}

Gelhaus, Christoph ^{[1
]}

机构：

[1] Univ Kiel, Inst Zool, Dept Zoophysiol, D-24098 Kiel, Germany

来源：

BIOLOGICAL CHEMISTRY | 2008年 / 389卷 / 12期

关键词：

malaria; parasite; Plasmodium falciparum; SYTOX (R) Green dye; viability assay;

D O I：

10.1515/BC.2008.167

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A range of various assays to measure chemosusceptibility of Plasmodium falciparum have been described in the literature. As the screening of a plethora of compounds for antiplasmodial activity is urgently needed and becomes a constantly increasing routine analysis, a test system has to fulfill the following requirements: sensitivity, reliability, simplicity of performance, high-throughput compatibility, and cost-effectiveness. Here, we describe an assay that fulfills all criteria and in which the fluorescent SYTOX (R) Green dye is introduced to determine growth inhibition of Plasmodia in in vitro cultures.

引用

页码：1523 / 1525

页数：3

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