An efficient fluorimetric method to measure the viability of intraerythrocytic Plasmodium falciparum

被引：12
|
作者：
Evers, Astrid [1 ]
Heppner, Saskia [1 ]
Leippe, Matthias [1 ]
Gelhaus, Christoph [1 ]
机构：
[1] Univ Kiel, Inst Zool, Dept Zoophysiol, D-24098 Kiel, Germany
来源：
BIOLOGICAL CHEMISTRY | 2008年 / 389卷 / 12期
关键词：
malaria; parasite; Plasmodium falciparum; SYTOX (R) Green dye; viability assay;
D O I：
10.1515/BC.2008.167
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A range of various assays to measure chemosusceptibility of Plasmodium falciparum have been described in the literature. As the screening of a plethora of compounds for antiplasmodial activity is urgently needed and becomes a constantly increasing routine analysis, a test system has to fulfill the following requirements: sensitivity, reliability, simplicity of performance, high-throughput compatibility, and cost-effectiveness. Here, we describe an assay that fulfills all criteria and in which the fluorescent SYTOX (R) Green dye is introduced to determine growth inhibition of Plasmodia in in vitro cultures.
引用
收藏
页码：1523 / 1525
页数：3
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