Peptidomic analysis of zebrafish embryos exposed to polychlorinated biphenyls and their impact on eye development

被引：8

作者：

Jiang, Yue ^{[1
]}

Zhang, Shuchun ^{[1
]}

Zhang, Xin ^{[1
,2
]}

Li, Nan ^{[3
]}

Zhang, Qingyu ^{[1
]}

Guo, Xirong ^{[1
,2
]}

Chi, Xia ^{[1
,2
]}

Tong, Meiling ^{[1
,2
]}

机构：

[1] Nanjing Med Univ, Dept Pediat, Nanjing 210004, Jiangsu, Peoples R China

[2] Nanjing Med Univ, Obstet & Gynecol Hosp, Nanjing Matern & Child Hlth Care Hosp, Dept Pediat, Nanjing, Jiangsu, Peoples R China

[3] Zhejiang Univ, Ningbo Hosp, Ningbo Hosp 1, Ningbo 315010, Zhejiang, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2019年 / 175卷

基金：

中国国家自然科学基金;

关键词：

Polychlorinated biphenyls; Zebrafish; Peptide; Ocular development; LC-MS/MS; PRENATAL EXPOSURE; ABNORMALITIES; EXPRESSION; SERUM; PCBS;

D O I：

10.1016/j.ecoenv.2019.03.015

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Polychlorinated biphenyls (PCBs), a class of persistent organic pollutant, are closely related to abnormal eye development in children. However, little is known regarding the role of peptides in the development of PCBinduced ocular dysplasia. To characterize the nature of PCB exposure on peptides involved in the development of the ocular system, we used liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) to detect differential expression of peptides between normal and PCB-exposed zebrafish embryos. A total of 7900 peptides were analyzed, 90 of which were differentially expressed, with 29 being up-regulated and 61 down-regulated. These peptides were investigated using ingenuity pathway analysis (IPA) and gene ontology (GO) analysis to explore their role in eye development. This study identified 18 peptides associated with the development of the optic nerve and ocular system in the PCB-exposure group, as well as 10 peptides that are located in the functional domain of their precursor proteins. These peptides provide potential biomarkers for the treatment of ocular dysplasia caused by PCBs and may help us understand the mechanism of abnormal eye development caused by organic pollutants.

引用

页码：164 / 172

页数：9

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