Adipogenic differentiation of murine bone marrow mesenchymal stem cells induced by visible light via photo- induced biomodulation

被引：11

作者：

Castilho-Fernandes, Andrielle ^{[1
]}

Lopes, Tadla G. ^{[1
]}

Ferreira, Fernando U. ^{[2
,3
]}

Rezende, Nayara ^{[1
]}

Silva, Valeria F. ^{[1
,2
,3
]}

Primo, Fernando L. ^{[1
,5
]}

Fontes, Aparecida Maria ^{[6
]}

Ribeiro-Silva, Alfredo ^{[4
]}

Tedesco, Antonio Claudio ^{[1
]}

机构：

[1] Univ Sao Paulo, Fac Philosophy Sci & Letters Ribeirao Preto, Ctr Nanotechnol & Tissue Engn, Dept Chem,Photobiol & Photomed Res Grp, BR-14040901 Ribeirao Preto, Brazil

[2] Univ Sao Paulo, Ctr Cell Therapy, Med Sch, Natl Inst Sci & Technol Stem Cell & Cell Therapy, BR-14051140 Ribeirao Preto, Brazil

[3] Univ Sao Paulo, Reg Blood Ctr, Med Sch, Natl Inst Sci & Technol Stem Cell & Cell Therapy, BR-14051140 Ribeirao Preto, Brazil

[4] Univ Sao Paulo, Med Sch, Dept Pathol, BR-14049900 Ribeirao Preto, Brazil

[5] Sao Paulo State Univ UNESP, Sch Pharmaceut Sci, Dept Bioproc & Biotechnol, Araraquara SP 14800-903, BR-14800903 Araraquara, SP, Brazil

[6] Univ Sao Paulo, Med Sch, Dept Genet, BR-14051140 Ribeirao Preto, Brazil

来源：

PHOTODIAGNOSIS AND PHOTODYNAMIC THERAPY | 2019年 / 25卷

基金：

巴西圣保罗研究基金会;

关键词：

Bone marrow-mesenchymal stem cells (BM-MSC); Photo induced biomodulation; Low level laser (LLL); Chloroaluminum phthalocyanine (AlClPc); Adipogenic differentiation; INTENSITY LASER IRRADIATION; STROMAL CELLS; PROLIFERATION; THERAPY; EXPRESSION; PHOTOBIOMODULATION; NANOEMULSION; ACTIVATION; SURVIVAL; GROWTH;

D O I：

10.1016/j.pdpdt.2018.11.013

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Background: Bone marrow mesenchymal stem cells (BM-MSCs) are undifferentiated cells that can proliferate and differentiate into specialized cells for tissue self-repair. Low-level laser (LLL) can induce biomodulatory effects such as cellular proliferation, differentiation, and migration. We investigated the biomodulatory effects of the photoactive compound chloroaluminum phthalocyanine nanoemulsion (AlClPc/NE) on the adipogenic differentiation of BM-MSCs, when combined with LLL (AlClPc/NE-LLL). Methods: The BM-MSCs used in this work were isolated from green fluorescent protein-positive (GFP(+)) C57BL6 mice. Cells were first treated with AlClPc/NE, a well-designed photoactive nano-drug and were then subjected to in vitro expansion, morphological and immunophenotypic characterization, and cellular cytotoxicity analysis. Subsequently, BM-MSCs were induced to differentiate into adipocytes by photo-induced biomodulation with AlClPc/NE-LLL. Results: Our results showed that the isolated cell population was consistent with murine BM-MSCs. The cellular cytotoxicity analysis revealed that the optimal nanoemulsion dose to induce BM-MSC biomodulation was 5.0 mu mol/L. Twenty-four hours following treatment with AlClPc/NE, BM-MSC were subjected to visible light irradiation of 20 mJ/cm(2) at 670 nm. Six days after photo-induced biomodulation, cells maintained high GFP expression level, and expressed detectable mRNA levels of adipogenic genes (lipoprotein lipase and PPAR gamma); formation of lipid vacuoles was observed, and the cells did not show any tumorigenic potential in vivo. Conclusions: Our results indicated that photo-induced biomodulation via visible light using AlClPc/NE and LLL can induce adipogenic differentiation of murine BM-MSCs. Therefore, cell therapy with BM-MSCs and photoinduced biomodulation may contribute to the development of new therapeutic strategies that are faster and more effective than traditional methods to trigger MSC differentiation.

引用

页码：119 / 127

页数：9

共 51 条

[21] The many facets of PPARγ: novel insights for the skeleton [J].