Fluorescent proteins as markers in the plant secretory pathway

被引：12

作者：

Hanton, SL ^{[1
]}

Brandizzi, F ^{[1
]}

机构：

[1] Univ Saskatchewan, Dept Biol, Saskatoon, SK S7N 5E2, Canada

来源：

MICROSCOPY RESEARCH AND TECHNIQUE | 2006年 / 69卷 / 03期

关键词：

plant secretory pathway; fluorescent protein; protein transport;

D O I：

10.1002/jemt.20276

中图分类号：

R602 [外科病理学、解剖学]; R32 [人体形态学];

学科分类号：

100101 ;

摘要：

The use of fluorescent proteins and live cell imaging has greatly increased our knowledge of cell biology in recent years. Not only can these technologies be used to study protein trafficking under different conditions, but they have also been of use in elucidating the relationships between different organelles in a noninvasive manner. The use of multiple different fluorochromes allows the observation of interactions between organelles and between proteins, making this one of the fastest-developing and exciting fields at this time. In this review, we discuss the multitude of fluorescent markers that have been generated to study the plant secretory pathway. Although these markers have been used to solve many mysteries in this field, some areas that require further discussion remain.

引用

页码：152 / 159

页数：8

共 92 条

[11] Membrane protein transport between the endoplasmic reticulum and the golgi in tobacco leaves is energy dependent but cytoskeleton independent: Evidence from selective photobleaching [J].

Brandizzi, F ;

Snapp, EL ;

Roberts, AG ;

Lippincott-Schwartz, J ;

Hawes, C .

PLANT CELL, 2002, 14 (06) :1293-1309

[12] The plant nuclear envelope: new prospects for a poorly understood structure [J].

Brandizzi, F ;

Irons, SL ;

Evans, DE .

NEW PHYTOLOGIST, 2004, 163 (02) :227-246

[13] The destination for single-pass membrane proteins is influenced markedly by the length of the hydrophobic domain [J].

Brandizzi, F ;

Frangne, N ;

Marc-Martin, S ;

Hawes, C ;

Neuhaus, JM ;

Paris, N .

PLANT CELL, 2002, 14 (05) :1077-1092

[14] A monomeric red fluorescent protein [J].

Campbell, RE ;

Tour, O ;

Palmer, AE ;

Steinbach, PA ;

Baird, GS ;

Zacharias, DA ;

Tsien, RY .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (12) :7877-7882

[15] GREEN FLUORESCENT PROTEIN AS A MARKER FOR GENE-EXPRESSION [J].

CHALFIE, M ;

TU, Y ;

EUSKIRCHEN, G ;

WARD, WW ;

PRASHER, DC .

SCIENCE, 1994, 263 (5148) :802-805

[16]

Conceicao ADS, 1997, PLANT CELL, V9, P571, DOI 10.2307/3870508

[17] FACS-optimized mutants of the green fluorescent protein (GFP) [J].

Cormack, BP ;

Valdivia, RH ;

Falkow, S .

GENE, 1996, 173 (01) :33-38

[18] Saturation of the endoplasmic reticulum retention machinery reveals anterograde bulk flow [J].

Crofts, AJ ;

Leborgne-Castel, N ;

Hillmer, S ;

Robinson, DG ;

Phillipson, B ;

Carlsson, LE ;

Ashford, DA ;

Denecke, J .

PLANT CELL, 1999, 11 (11) :2233-2247

[19] Receptor salvage from the prevacuolar compartment is essential for efficient vacuolar protein targeting [J].

daSilva, LLP ;

Taylor, JP ;

Hadlington, JL ;

Hanton, SL ;

Snowden, CJ ;

Fox, SJ ;

Foresti, O ;

Brandizzi, F ;

Denecke, J .

PLANT CELL, 2005, 17 (01) :132-148

[20] Endoplasmic reticulum export sites and golgi bodies behave as single mobile secretory units in plant cells [J].

DaSilva, LLP ;

Snapp, EL ;

Denecke, J ;

Lippincott-Schwartz, J ;

Hawes, C ;

Brandizzi, F .

PLANT CELL, 2004, 16 (07) :1753-1771

← 1 2 3 4 5 6 7 8 9 10 →