Trypanosoma brucei Tb927.2.6100 Is an Essential Protein Associated with Kinetoplast DNA

被引：22

作者：

Beck, Kirsten ^{[1
]}

Acestor, Nathalie ^{[1
]}

Schulfer, Anjelique ^{[1
]}

Anupama, Atashi ^{[1
]}

Carnes, Jason ^{[1
]}

Panigrahi, Aswini K. ^{[2
]}

Stuart, Ken ^{[1
]}

机构：

[1] Seattle Biomed Res Inst, Seattle, WA 98109 USA

[2] King Abdullah Univ Sci & Technol, Thuwal, Saudi Arabia

来源：

EUKARYOTIC CELL | 2013年 / 12卷 / 07期

基金：

美国国家卫生研究院;

关键词：

BLOOD-STREAM FORM; MITOCHONDRIAL RNA-POLYMERASE; CRITHIDIA-LUCILIAE; STATISTICAL-MODEL; BINDING COMPLEX; GENE-EXPRESSION; REPLICATION; NETWORK; INTERFERENCE; TIME;

D O I：

10.1128/EC.00352-12

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The mitochondrial DNA of trypanosomatid protozoa consists of a complex, intercatenated network of tens of maxicircles and thousands of minicircles. This structure, called kinetoplast DNA (kDNA), requires numerous proteins and multiprotein complexes for replication, segregation, and transcription. In this study, we used a proteomic approach to identify proteins that are associated with the kDNA network. We identified a novel protein encoded by Tb927.2.6100 that was present in a fraction enriched for kDNA and colocalized the protein with kDNA by fluorescence microscopy. RNA interference (RNAi) knockdown of its expression resulted in a growth defect and changes in the proportion of kinetoplasts and nuclei in the cell population. RNAi also resulted in shrinkage and loss of the kinetoplasts, loss of maxicircle and minicircle components of kDNA at similar rates, and (perhaps secondarily) loss of edited and pre-edited mRNA. These results indicate that the Tb927.2.6100 protein is essential for the maintenance of kDNA.

引用

页码：970 / 978

页数：9

共 58 条

[1] The MRB1 complex functions in kinetoplastid RNA processing [J].