Effects of the prebiotic inulin-type fructans on post-antibiotic reconstitution of the gut microbiome

被引:7
作者
Tao, C. [1 ]
Zeng, W. [1 ]
Zhang, Q. [1 ]
Liu, G. [2 ]
Wu, F. [1 ]
Shen, H. [1 ]
Zhang, W. [1 ]
Bo, H. [1 ]
Shao, H. [1 ]
机构
[1] Guangdong Pharmaceut Univ, Sch Biosci & Biopharmaceut, Guangdong Prov Key Lab Biotechnol Drug Candidates, Guangzhou 510006, Peoples R China
[2] Zhongkai Univ Agr & Engn, Coll Light Ind & Food Sci, Guangzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
antibiotic cocktail (ABx); gut microbiota; inulin-type fructans (ITFs); prebiotic; reconstitution; GALACTO-OLIGOSACCHARIDES; LIPID PROFILE; PROBIOTICS; HOST; SUPPLEMENTATION; BIFIDOBACTERIA; SYNBIOTICS; SEQUENCES; RESPONSES; BUTYRATE;
D O I
10.1111/jam.14827
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Aims Interventions using prebiotic inulin-type fructans (ITFs) are widely prescribed to modulate the gut microbiota composition and activity to promote health. However, the impacts of ITFs on post-antibiotic reconstitution of the gut microbiome remain incompletely understood. The aim of the present study was to investigate the effects of ITFs supplementation on intestinal inflammation, the composition of the intestinal microbiota and the colonic transcriptome after antibiotic treatment. Methods and Results Male BALB/c mice were subjected to an antibiotic cocktail (ABx) treatment for 7 days, and their microbiomes were then reconstituted either spontaneously or with ITFs supplementation (5%) for 14 days. Our data showed that ITFs supplementation delayed the recovery of antibiotic-induced colitis compared with the spontaneous recovery. Neither ITFs supplementation nor spontaneous recovery could restore the microbial community composition at the genus level back to its initial composition. ITFs supplementation increased the relative abundance of some beneficial bacteria and butyrate levels, but resulted in selective blooms of some opportunistic pathogens and elevated the pathways associated with diseases linked to gut microbiota function. Both ITFs supplementation and spontaneous recovery could restore the colonic transcriptome nearly to the initial profile to a certain extent; however, ITFs supplementation delayed the restoration of the immunoglobulin genes compared to spontaneous recovery. Conclusion These data showed that post-antibiotic ITFs consumption did not always lead to beneficial effects but might lead to potential adverse effects in the context of dysbiosis. Significance and Impact of the Study These findings highlighted that caution is required when supplementing ITFs to restore intestinal homeostasis in the context of dysbiosis resulting from broad-spectrum antibiotics.
引用
收藏
页码:634 / 649
页数:16
相关论文
共 51 条
  • [1] Antibiotic use and its consequences for the normal microbiome
    Blaser, Martin J.
    [J]. SCIENCE, 2016, 352 (6285) : 544 - 545
  • [2] Commensal Bacteroides Species Induce Colitis in Host-Genotype-Specific Fashion in a Mouse Model of Inflammatory Bowel Disease
    Bloom, Seth M.
    Bijanki, Vinieth N.
    Nava, Gerardo M.
    Sun, Lulu
    Malvin, Nicole P.
    Donermeyer, David L.
    Dunne, W. Michael, Jr.
    Allen, Paul M.
    Stappenbeck, Thaddeus S.
    [J]. CELL HOST & MICROBE, 2011, 9 (05) : 390 - 403
  • [3] Trimmomatic: a flexible trimmer for Illumina sequence data
    Bolger, Anthony M.
    Lohse, Marc
    Usadel, Bjoern
    [J]. BIOINFORMATICS, 2014, 30 (15) : 2114 - 2120
  • [4] Fast and sensitive protein alignment using DIAMOND
    Buchfink, Benjamin
    Xie, Chao
    Huson, Daniel H.
    [J]. NATURE METHODS, 2015, 12 (01) : 59 - 60
  • [5] QIIME allows analysis of high-throughput community sequencing data
    Caporaso, J. Gregory
    Kuczynski, Justin
    Stombaugh, Jesse
    Bittinger, Kyle
    Bushman, Frederic D.
    Costello, Elizabeth K.
    Fierer, Noah
    Pena, Antonio Gonzalez
    Goodrich, Julia K.
    Gordon, Jeffrey I.
    Huttley, Gavin A.
    Kelley, Scott T.
    Knights, Dan
    Koenig, Jeremy E.
    Ley, Ruth E.
    Lozupone, Catherine A.
    McDonald, Daniel
    Muegge, Brian D.
    Pirrung, Meg
    Reeder, Jens
    Sevinsky, Joel R.
    Tumbaugh, Peter J.
    Walters, William A.
    Widmann, Jeremy
    Yatsunenko, Tanya
    Zaneveld, Jesse
    Knight, Rob
    [J]. NATURE METHODS, 2010, 7 (05) : 335 - 336
  • [6] Lactobacillus reuteri induces gut intraepithelial CD4+CD8αα+ T cells
    Cervantes-Barragan, Luisa
    Chai, Jiani N.
    Tianero, Ma. Diarey
    Di Luccia, Blanda
    Ahern, Philip P.
    Merriman, Joseph
    Cortez, Victor S.
    Caparon, Michael G.
    Donia, Mohamed S.
    Gilfillan, Susan
    Cella, Marina
    Gordon, Jeffrey I.
    Hsieh, Chyi-Song
    Colonna, Marco
    [J]. SCIENCE, 2017, 357 (6353) : 806 - 810
  • [7] Specific inulin-type fructan fibers protect against autoimmune diabetes by modulating gut immunity, barrier function, and microbiota homeostasis
    Chen, Kang
    Chen, Hao
    Faas, Marijke M.
    de Haan, Bart J.
    Li, Jiahong
    Xiao, Ping
    Zhang, Hao
    Diana, Julien
    de Vos, Paul
    Sun, Jia
    [J]. MOLECULAR NUTRITION & FOOD RESEARCH, 2017, 61 (08)
  • [8] Decreased microglial activation through gut-brain axis by prebiotics, probiotics, or synbiotics effectively restored cognitive function in obese-insulin resistant rats
    Chunchai, Titikorn
    Thunapong, Wannipa
    Yasom, Sakawdaurn
    Wanchai, Keerati
    Eaimworawuthikul, Sathima
    Metzler, Gabrielle
    Lungkaphin, Anusorn
    Pongchaidecha, Anchalee
    Sirilun, Sasithorn
    Chaiyasut, Chaiyavat
    Pratchayasakul, Wasana
    Thiennimitr, Parameth
    Chattipakorn, Nipon
    Chattipakorn, Siriporn C.
    [J]. JOURNAL OF NEUROINFLAMMATION, 2018, 15
  • [9] The use of inulin-type fructans improves stool consistency in constipated children. A randomised clinical trial: pilot study
    Closa-Monasterolo, Ricardo
    Ferre, Natalia
    Castillejo-DeVillasante, Gemma
    Luque, Veronica
    Gispert-Llaurado, Mariona
    Zaragoza-Jordana, Marta
    Theis, Stephan
    Escribano, Joaquin
    [J]. INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 2017, 68 (05) : 587 - 594
  • [10] The mucin degrader Akkermansia muciniphila is an abundant resident of the human intestinal tract
    Derrien, Muriel
    Collado, M. Carmen
    Ben-Amor, Kaouther
    Salminen, Seppo
    de Vos, Willem M.
    [J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2008, 74 (05) : 1646 - 1648