Spectroscopic Determination of Lysozyme Conformational Changes in the Presence of Trehalose and Guanidine

被引：7

作者：

Barreca, Davide ^{[1
]}

Lagana, Giuseppina ^{[1
]}

Ficarra, Silvana ^{[1
]}

Gattuso, Giuseppe ^{[1
]}

Magazu, Salvatore ^{[2
]}

La Torre, Roberto ^{[2
]}

Tellone, Ester ^{[1
]}

Bellocco, Ersilia ^{[1
]}

机构：

[1] Univ Messina, Dept Organ & Biol Chem, I-98166 Messina, Italy

[2] Univ Messina, Dept Phys, I-98166 Messina, Italy

来源：

CELL BIOCHEMISTRY AND BIOPHYSICS | 2013年 / 66卷 / 02期

关键词：

Trehalose; Lysozyme; ESI-MS hydrogen-deuterium exchange; Circular dichroism; H-1; NMR; EGG-WHITE LYSOZYME; NEUTRON-SCATTERING; DYNAMICS;

D O I：

10.1007/s12013-012-9485-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The bioprotective action of the disaccharide trehalose has been studied against the well-known denaturating agent, guanidine hydrochloride. The results indicated a direct influence of trehalose on both enzymatic activity and conformational changes of lysozyme, as shown by the decrease of the inactivation rate constant of about 1.48-fold and the loss of alpha-helix structure of lysozyme. In addition, ESI-MS hydrogen-deuterium (H/D) exchange experiments allowed us to correlate the structural and dynamic features of the protein in the presence of the two additives, highlighting as trehalose remarkably influenced this exchange by decreasing local protein environment changes and solvent accessibility to the amide peptide backbone, as further evidenced by circular dichroism and H-1 NMR measurements.

引用

页码：297 / 307

页数：11

共 22 条

[1] Spectroscopic investigation of structure-breakers and structure-makers on ornithine carbamoyltransferase [J].

Barreca, D. ;

Bellocco, E. ;

Lagana, G. ;

Leuzzi, U. ;

Magazù, S. ;

Migliardo, F. ;

Galtlieri, A. .

FOOD CHEMISTRY, 2008, 106 (04) :1438-1442

[2] Stabilization effects of kosmotrope systems on ornithine carbamoyltransferase [J].

Barreca, D. ;

Bellocco, E. ;

Galli, G. ;

Lagana, G. ;

Leuzzi, U. ;

Magazu, S. ;

Migliardo, F. ;

Galtieri, A. ;

Telling, Mark T. F. .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2009, 45 (02) :120-128

[3] Anti-aggregation properties of trehalose on heat-induced secondary structure and conformation changes of bovine serum albumin [J].

Barreca, Davide ;

Lagana, Giuseppina ;

Ficarra, Silvana ;

Tellone, Ester ;

Leuzzi, Ugo ;

Magazu, Salvatore ;

Galtieri, Antonio ;

Bellocco, Ersilia .

BIOPHYSICAL CHEMISTRY, 2010, 147 (03) :146-152

[4]

Batas D, 1996, BIOTECHNOL BIOENG, V50, P16

[5] Neutron scattering and HPLC study on L-ascorbic acid and its degradation [J].

Bellocco, E. ;

Barreca, D. ;

Lagana, G. ;

Leuzzi, U. ;

Migliardo, F. ;

La Torre, R. ;

Galli, G. ;

Galtleri, A. ;

Minutoli, L. ;

Squadrito, F. .

CHEMICAL PHYSICS, 2008, 345 (2-3) :191-195

[6] Role of polyols in thermal inactivation of shark ornithine transcarbamoylase [J].

Bellocco, E ;

Laganà, G ;

Barreca, D ;

Ficarra, S ;

Tellone, E ;

Magazù, S ;

Branca, C ;

Kotyk, A ;

Galtieri, A ;

Leuzzi, U .

PHYSIOLOGICAL RESEARCH, 2005, 54 (04) :395-402

[7] Influences of temperature and threshold effect of NaCl concentration on Alpias vulpinus OCT [J].

Bellocco, Ersila ;

Barreca, Davide ;

Lagana, Giuseppina ;

Tellone, Ester ;

Ficarra, Silvana ;

Migliardo, Federica ;

Leuzzi, Ugo ;

Magazu, Salvatore ;

Galtieri, Antonio .

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2008, 43 (05) :474-480

[8] CIRCULAR DICHROSIM OF BIOLOGICAL MACROMOLECULES [J].

BEYCHOK, S .

SCIENCE, 1966, 154 (3754) :1288-&

[9] Comparative study of the thermostabilizing properties of mannosylglycerate and other compatible solutes on model enzymes [J].

Borges, N ;

Ramos, A ;

Raven, NDH ;

Sharp, RJ ;

Santos, H .

EXTREMOPHILES, 2002, 6 (03) :209-216

[10] Insights into enzyme structure and dynamics elucidated by amide H/D exchange mass spectrometry [J].

Busenlehner, LS ;

Armstrong, RN .

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2005, 433 (01) :34-46

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