Side-by-side comparison of disposable microchips with commercial capillary cartridges for application in capillary isoelectric focusing with whole column imaging detection

被引：17

作者：

Liu, Zhen ^{[1
]}

On, Junjie ^{[2
,3
]}

Samy, Razim ^{[2
]}

Glawdel, Tomasz ^{[2
]}

Huang, Tiemin ^{[4
]}

Ren, Carolyn L. ^{[2
]}

Pawliszyn, Janusz ^{[3
]}

机构：

[1] Nanjing Univ, Sch Chem & Chem Engn, Nanjing 210093, Peoples R China

[2] Univ Waterloo, Dept Mech & Mechatron Engn, Waterloo, ON N2L 3G1, Canada

[3] Univ Waterloo, Dept Chem, Waterloo, ON N2L 3G1, Canada

[4] Convergent Biosci Ltd, Toronto, ON M8Z 2L8, Canada

来源：

LAB ON A CHIP | 2008年 / 8卷 / 10期

基金：

中国国家自然科学基金; 加拿大自然科学与工程研究理事会;

关键词：

D O I：

10.1039/b807646d

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Simple-structured, well-functioned disposable poly(dimethylsiloxane) (PDMS) microchips were developed for capillary isoelectric focusing with whole column imaging detection (CIEF-WCID). Side-by-side comparison of the developed microchips with well-established commercial capillary cartridges demonstrated that the disposable microchips have comparable performance as well as advantages such as absence of lens effect and possibility of high-aspect-ratio accompanied with a dramatic reduction in cost.

引用

页码：1738 / 1741

页数：4

共 20 条

[1] Isoelectric focusing in a poly(dimethylsiloxane) microfluidic chip
Cui, HC
Horiuchi, K
Dutta, P
Ivory, CF
[J]. ANALYTICAL CHEMISTRY, 2005, 77 (05) : 1303 - 1309
[2] Effects of separation length and voltage on isolectric focusing in a plastic microfluidic device
Das, Champak
Fan, Z. Hugh
[J]. ELECTROPHORESIS, 2006, 27 (18) : 3619 - 3626
[3] Rapid prototyping of microfluidic systems in poly(dimethylsiloxane)
Duffy, DC
McDonald, JC
Schueller, OJA
Whitesides, GM
[J]. ANALYTICAL CHEMISTRY, 1998, 70 (23) : 4974 - 4984
[4] Isoelectric point determination of norovirus virus-like particles by capillary Isoelectric focusing with whole column imaging detection
Goodridge, L
Goodridge, C
Wu, JQ
Griffiths, M
Pawliszyn, J
[J]. ANALYTICAL CHEMISTRY, 2004, 76 (01) : 48 - 52
[5] Jochheim C, 2001, CHROMATOGRAPHIA, V53, pS59
[6] Capillary isoelectric focusing coupled with dynamic imaging detection: A one-dimensional separation for two-dimensional protein characterization
Liu, Z
Lemma, T
Pawliszyn, J
[J]. JOURNAL OF PROTEOME RESEARCH, 2006, 5 (05) : 1246 - 1251
[7] Capillary isoelectric focusing with laser-induced fluorescence whole column imaging detection as a tool to monitor reactions of proteins
Liu, Z
Pawliszyn, J
[J]. JOURNAL OF PROTEOME RESEARCH, 2004, 3 (03) : 567 - 571
[8] Capillary isoelectric focusing of proteins with liquid core waveguide laser-induced fluorescence whole column imaging detection
Liu, Z
Pawliszyn, J
[J]. ANALYTICAL CHEMISTRY, 2003, 75 (18) : 4887 - 4894
[9] Characterization of plant growth-promoting rhizobacteria using capillary isoelectric focusing with whole column imaging detection
Liu, Zhen
Wu, Shan Shan
Pawliszyn, Janusz
[J]. JOURNAL OF CHROMATOGRAPHY A, 2007, 1140 (1-2) : 213 - 218
[10] Dynamic kinetic capillary isoelectric focusing: A powerful tool for studying protein-DNA interactions
Liu, Zhen
Drabovich, Andrei P.
Krylov, Sergey N.
Pawliszyn, Janusz
[J]. ANALYTICAL CHEMISTRY, 2007, 79 (03) : 1097 - 1100

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