Enhancement of Biogas Production in Anaerobic Digestion Using Microbial Electrolysis Cell Seed Sludge

Anaerobic digestion (AD) can produce renewable energy and reduce carbon emissions, but the energy conversion efficiency is still limited in some waste streams. This study tested the effect of applied voltage removal for microbial electrolysis cells (MECs) treating primary sewage sludge. Two MECs were operated in parallel: a MEC-0.3 V with an applied voltage of 0.3 V and a MEC-OCV with open circuit voltage. Both reactors were inoculated with seed sludge originating from a MEC at 0.3 V applied voltage, and three batch cycles were operated for 36 d. The methane production of the MEC-OCV was 3759 mL/L in the first cycle and 2759 mL/L in the second cycle, which was similar (105% and 103%, respectively) to that of the MEC-0.3 V. However, in the third cycle, the methane production of the MEC-OCV (1762 mL/L) was 38.8% lower than that of the MEC-0.3 V (4545 mL/L). The methane contents in the biogas were 68.6-74.2% from the MEC-OCV, comparable to those from the MEC-0.3 V (66.6-71.1%). These results indicate that not only the MEC-0.3V but also the MEC-OCV outperformed AD in terms of methane yield and productivity, and the promotion using MEC-derived inoculum persisted equally with the MEC-OCV for two batch cycles after removing the applied voltage. Therefore, a MEC operation with cycled power supply may be beneficial in reducing the electric energy usage and improving the biogas production performance, compared to conventional AD.