Comparison of the Separation Selectivity of Purine Derivatives in High-Performance Thin-Layer Chromatography and Pressurized Planar Electrochromatography Systems

被引：1

作者：

Gwarda, Radoslaw Lukasz ^{[1
]}

Halka-Grysinska, Aneta ^{[1
]}

Plocharz, Pawel Wladyslaw ^{[2
]}

Stadniczenko, Anna ^{[1
]}

Dzido, Tadeusz Henryk ^{[1
]}

机构：

[1] Med Univ Lublin, Dept Phys Chem, Chair Chem, 4a Chodzki St, PL-20093 Lublin, Poland

[2] Jozef Pilsudski Univ Phys Educ Warsaw, Fac Phys Educ & Sport Biala Podlaska, Dept Cosmetol, 2 Akad Str, PL-21500 Biala Podlaska, Poland

来源：

JPC-JOURNAL OF PLANAR CHROMATOGRAPHY-MODERN TLC | 2018年 / 31卷 / 01期

关键词：

Pressurized planar electrochromatography; Thin-layer chromatography; Purine derivatives; Comparison of separation selectivity; LIQUID-CHROMATOGRAPHY; SAMPLE APPLICATION; MASS-SPECTROMETRY; CLOSED-SYSTEM; UPLC-MS/MS; TEA; HPLC; METABOLITES; CAFFEINE; METHYLXANTHINES;

D O I：

10.1556/1006.2018.31.1.7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Our article presents the comparison of two methods: high-performance thin-layer chromatography (HPTLC) and pressurized planar electrochromatography (PPEC), implemented for the separation of a test mixture of purine derivatives. The two methods were compared in terms of separation selectivity and separation time. Our results show that PPEC enables the separation of the mixture (azathioprine, caffeine, theobromine, theophylline and acyclovir) which could not be efficiently separated in the HPTLC system, due to the different selectivities of separation. PPEC also enables to obtain a much faster separation, performed on the longer distance, in comparison to HPTLC. This makes PPEC a technique which can be useful in the analysis of purine derivatives and other drugs.

引用

页码：57 / 60

页数：4

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