Detection of InDel and CNV ofSPAG17gene and their associations with bovine growth traits

被引：12

作者：

Guo, Xian ^{[1
,2
]}

Pei, Jie ^{[1
,2
]}

Wu, Xiaoyun ^{[1
,2
]}

Bao, Pengjia ^{[1
,2
]}

Ding, Xuezhi ^{[1
,2
]}

Xiong, Lin ^{[1
,2
]}

Chu, Min ^{[1
,2
]}

Lan, Xianyong ^{[3
]}

Yan, Ping ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Inst Husb & Pharmaceut Sci, Lanzhou 730050, Gansu, Peoples R China

[2] Key Lab Yak Breeding Engn Gansu Prov, Lanzhou, Gansu, Peoples R China

[3] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling, Shaanxi, Peoples R China

来源：

ANIMAL BIOTECHNOLOGY | 2022年 / 33卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Cattle; SPAG17; insertions; deletions (indels); copy number variations (CNVs); growth traits; COPY NUMBER VARIATIONS; TRANSCRIPTIONAL EXPRESSION; GENE; HEIGHT; GENOME; CATTLE;

D O I：

10.1080/10495398.2020.1803342

中图分类号：

S8 [畜牧、动物医学、狩猎、蚕、蜂];

学科分类号：

0905 ;

摘要：

Sperm-associated antigen 17 (SPAG17) gene encodes a central pair protein, which is involved in flagellar motility, male fertility and skeletal growth in ruminants. The insertions/deletions (indels) and copy number variations (CNVs) influence phenotypic traits by altering the sequences and copy numbers of functional genes, respectively. This study identified a novel 8-bp indel ofSPAG17gene in 1520 individuals from eight different cattle breeds, as well as a novel CNV region in 355 animals. The correlation analysis of indel showed that the individuals of ID genotype had superior performance traits such as body height (p = 0.038) and body slanting length (p = 0.041) as compared to other genotypes in Xianan cattle. For the CNV, different copy numbers were closely related to the body height in Qinchuan (p = 0.045) and body weight in Xianan (p = 0.036) breeds. Importantly, significant difference was observed between the 8-bp indel and the copy number loss in Xianan breed (p < 0.01). These findings indicated that the variations within the bovineSPAG17gene can be considered as an effective DNA molecular marker for beef cattle breeding.

引用

页码：440 / 447

页数：8

共 42 条

[11] Human beta defensin (HBD) gene copy number affects HBD2 protein levels: impact on cervical bactericidal immunity in pregnancy [J].

James, Catherine P. ;

Bajaj-Elliott, Mona ;

Abujaber, Razan ;

Forya, Frida ;

Klein, Nigel ;

David, Anna L. ;

Hollox, Edward J. ;

Peebles, Donald M. .

EUROPEAN JOURNAL OF HUMAN GENETICS, 2018, 26 (03) :434-439

[12] Novel genetic variants associated with mRNA expression of signal transducer and activator of transcription 3(STAT3) gene significantly affected goat growth traits [J].

Jia, Wenchao ;

Wu, Xianfeng ;

Li, Xiangchen ;

Xia, Tian ;

Lei, Chuzhao ;

Chen, Hong ;

Pan, Chuanying ;

Lan, Xianyong .

SMALL RUMINANT RESEARCH, 2015, 129 :25-36

[13] The 10 bp duplication insertion/deletion in the promoter region within paired box 7 gene is associated with growth traits in cattle [J].

Jin, Yunyun ;

Cai, Hanfang ;

Liu, Jiming ;

Lin, Fengpeng ;

Qi, Xinglei ;

Bai, Yueyu ;

Lei, Chuzhao ;

Chen, Hong ;

Lan, Xianyong .

ARCHIVES ANIMAL BREEDING, 2016, 59 (04) :469-476

[14] Copy number variation of bovine MAPK10 modulates the transcriptional activity and affects growth traits [J].

Liu, Mei ;

Li, Bo ;

Huang, Yongzhen ;

Yang, Mingjuan ;

Lan, Xianyong ;

Lei, Chuzhao ;

Qu, Weidong ;

Bai, Yueyu ;

Chen, Hong. .

LIVESTOCK SCIENCE, 2016, 194 :44-50

[15] Genome-wide copy number variant discovery in dogs using the CanineHD genotyping array [J].

Molin, Anna-Maja ;

Berglund, Jonas ;

Webster, Matthew T. ;

Lindblad-Toh, Kerstin .

BMC GENOMICS, 2014, 15

[16] Coding region structural heterogeneity and turnover of transcription start sites contribute to divergence in expression between duplicate genes [J].

Park, Chungoo ;

Makova, Kateryna D. .

GENOME BIOLOGY, 2009, 10 (01)

[17] Global variation in copy number in the human genome [J].

Redon, Richard ;

Ishikawa, Shumpei ;

Fitch, Karen R. ;

Feuk, Lars ;

Perry, George H. ;

Andrews, T. Daniel ;

Fiegler, Heike ;

Shapero, Michael H. ;

Carson, Andrew R. ;

Chen, Wenwei ;

Cho, Eun Kyung ;

Dallaire, Stephanie ;

Freeman, Jennifer L. ;

Gonzalez, Juan R. ;

Gratacos, Monica ;

Huang, Jing ;

Kalaitzopoulos, Dimitrios ;

Komura, Daisuke ;

MacDonald, Jeffrey R. ;

Marshall, Christian R. ;

Mei, Rui ;

Montgomery, Lyndal ;

Nishimura, Kunihiro ;

Okamura, Kohji ;

Shen, Fan ;

Somerville, Martin J. ;

Tchinda, Joelle ;

Valsesia, Armand ;

Woodwark, Cara ;

Yang, Fengtang ;

Zhang, Junjun ;

Zerjal, Tatiana ;

Zhang, Jane ;

Armengol, Lluis ;

Conrad, Donald F. ;

Estivill, Xavier ;

Tyler-Smith, Chris ;

Carter, Nigel P. ;

Aburatani, Hiroyuki ;

Lee, Charles ;

Jones, Keith W. ;

Scherer, Stephen W. ;

Hurles, Matthew E. .

NATURE, 2006, 444 (7118) :444-454

[18] Detection of breed-specific copy number variations in domestic chicken genome [J].

Sohrabi, Saeed S. ;

Mohammadabadi, Mohammadreza ;

Wu, Dong-Dong ;

Esmailizadeh, Ali .

GENOME, 2018, 61 (01) :7-14

[19] Spag17 Deficiency Results in Skeletal Malformations and Bone Abnormalities [J].

Teves, Maria Eugenia ;

Sundaresan, Gobalakrishnan ;

Cohen, David J. ;

Hyzy, Sharon L. ;

Kajan, Illya ;

Maczis, Melissa ;

Zhang, Zhibing ;

Costanzo, Richard M. ;

Zweit, Jamal ;

Schwartz, Zvi ;

Boyan, Barbara D. ;

Strauss, Jerome F., III .

PLOS ONE, 2015, 10 (05)

[20] Distribution and Functionality of Copy Number Variation across European Cattle Populations [J].

Upadhyay, Maulik ;

da Silva, Vinicus H. ;

Megens, Hendrik-Jan ;

Visker, Marleen H. P. W. ;

Ajmone-Marsan, Paolo ;

Balteanu, Valentin A. ;

Dunner, Susana ;

Garcia, Jose F. ;

Ginja, Catarina ;

Kantanen, Juha ;

Groenen, Martien A. M. ;

Crooijmans, Richard P. M. A. .

FRONTIERS IN GENETICS, 2017, 8

← 1 2 3 4 5 →