Extracellular signal-regulated kinase (ERK) activation is required for GP lbα-dependent endothelial cell migration

被引：4
作者：
Lian, J
Marcinkiewicz, C
Niewiarowski, S
Beacham, DA
机构：
[1] Temple Univ, Sch Med, Sol Sherry Thrombosis Res Ctr, Dept Physiol, Philadelphia, PA 19140 USA
[2] Thomas Jefferson Univ, Jefferson Med Coll, Cardeza Fdn Hematol Res, Philadelphia, PA USA
来源：
THROMBOSIS AND HAEMOSTASIS | 2001年 / 86卷 / 06期
关键词：
endothelial cell; migration; extracellular signal-regulated kinase; snake venom;
D O I：
10.1055/s-0037-1616762
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
The GP lb complex can participate in endothelial cell (EC) migration on von Willebrand factor (vWF) or the mixed matrix of vWF and type I collagen (vWF/collagen). In this study, viper venom proteins alboaggregin (albo) A or B blocked GP Ib alpha, and echistatin inhibited alphav beta3 binding. Albo A. B and echistatin inhibited EC migration on vWF and vWF/collagen. Albo B or the anti-GP Ib alpha monoclonal antibody (mAb) Ib1 did not affect the migration of smooth muscle cells or fibroblasts, which lack GP lb. EC also migrate on albo A- or albo B-coated dishes. PD98059. which blocks ERK activation, abolished EC migration on vWF, vWF/collagen, collagen or albo B. Soluble albo A or Ib1 dramatically inhibited ERK activation during EC migration on vWF or albo B. Echistatin inhibited ERK activation on vWF and vitronectin (VN), but not albo B. Thus. in addition to alphav beta3, EC GP Ib alpha initiates ERK activation, and regulates ERK-induced EC migration on vWF.
引用
收藏
页码：1555 / 1562
页数：8
相关论文
共 33 条
[21]   INTEGRIN BETA-1-MEDIATED AND BETA-3-MEDIATED ENDOTHELIAL-CELL MIGRATION IS TRIGGERED THROUGH DISTINCT SIGNALING MECHANISMS [J].
LEAVESLEY, DI ;
SCHWARTZ, MA ;
ROSENFELD, M ;
CHERESH, DA .
JOURNAL OF CELL BIOLOGY, 1993, 121 (01) :163-170
[22]   Glycoprotein Ibα can mediate endothelial cell migration on von Willebrand factor-containing substrata [J].
Lian, J ;
Cai, GP ;
Shapiro, SS ;
Tran, LP ;
Beacham, DA .
EXPERIMENTAL CELL RESEARCH, 1999, 252 (01) :114-122
[23]  
LOPEZ JA, 1994, BLOOD COAGUL FIBRIN, V5, P97
[24]   Structural and functional characterization of EMF10, a heterodimeric disintegrin from Eristocophis macmahoni venom that selectively inhibits α5β1 integrin [J].
Marcinkiewicz, C ;
Calvete, JJ ;
Vijay-Kumar, S ;
Marcinkiewicz, MM ;
Raida, M ;
Schick, P ;
Lobb, PR ;
Niewiarowski, S .
BIOCHEMISTRY, 1999, 38 (40) :13302-13309
[25]   Isolation and characterization of EMS16, a C-lectin type protein from Echis multisquamatus venom, a potent and selective inhibitor of the α2β1 integrin [J].
Marcinkiewicz, C ;
Lobb, RR ;
Marcinkiewicz, MM ;
Daniel, JL ;
Smith, JB ;
Dangelmaier, C ;
Weinreb, PH ;
Beacham, DA ;
Niewiarowski, S .
BIOCHEMISTRY, 2000, 39 (32) :9859-9867
[26]   Viper venom disintegrins and related molecules [J].
McLane, MA ;
Marcinkiewicz, C ;
Vijay-Kumar, S ;
Wierzbicka-Patynowski, I ;
Niewiarowski, S .
PROCEEDINGS OF THE SOCIETY FOR EXPERIMENTAL BIOLOGY AND MEDICINE, 1998, 219 (02) :109-119
[27]   Cloning and expression of the platelet-specific collagen receptor glycoprotein VI [J].
Miura, Y ;
Ohnuma, M ;
Jung, SM ;
Moroi, M .
THROMBOSIS RESEARCH, 2000, 98 (04) :301-309
[28]  
PENG ML, 1992, THROMB HAEMOSTASIS, V67, P702
[29]  
Shuster CB, 1998, MICROCIRCULATION, V5, P239
[30]   Identification and characterization of endothelial glycoprotein Ib using viper venom proteins modulating cell adhesion [J].
Tan, L ;
Kowalska, MA ;
Romo, GM ;
Lopez, JA ;
Darzynkiewicz, Z ;
Niewiarowski, S .
BLOOD, 1999, 93 (08) :2605-2616
1234