Safe Pseudovirus-based Assay for Neutralization Antibodies against Influenza A(H7N9) Virus

被引：39

作者：

Qiu, Chao ^{[1
,2
]}

Huang, Yang ^{[2
]}

Zhang, Anli ^{[1
,2
]}

Tian, Di ^{[1
]}

Wan, Yanmin ^{[1
]}

Zhang, Xiaoling ^{[1
]}

Zhang, Wanju ^{[1
]}

Zhang, Zhiyong ^{[1
]}

Yuan, Zhenghong ^{[1
,2
]}

Hu, Yunwen ^{[1
]}

Zhang, Xiaoyan ^{[1
,2
,3
]}

Xu, Jianqing ^{[1
,2
,3
]}

机构：

[1] Shanghai Publ Hlth Clin Ctr, Shanghai 201508, Peoples R China

[2] Fudan Univ, Shanghai 200433, Peoples R China

[3] Chinese Ctr Dis Control & Prevent, Beijing, Peoples R China

来源：

EMERGING INFECTIOUS DISEASES | 2013年 / 19卷 / 10期

关键词：

D O I：

10.3201/eid1910.130728

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Serologic studies are urgently needed to assist in understanding an outbreak of influenza A(H7N9) virus. However, a biosafety level 3 laboratory is required for conventional serologic assays with live lethal virus. We describe a safe pseudovirus based neutralization assay with preliminary assessment using subtype H7N9-infected samples and controls.

引用

页码：1685 / 1687

页数：3

共 7 条

[1] Human Infection with a Novel Avian-Origin Influenza A (H7N9) Virus [J].

Gao, Rongbao ;

Cao, Bin ;

Hu, Yunwen ;

Feng, Zijian ;

Wang, Dayan ;

Hu, Wanfu ;

Chen, Jian ;

Jie, Zhijun ;

Qiu, Haibo ;

Xu, Ke ;

Xu, Xuewei ;

Lu, Hongzhou ;

Zhu, Wenfei ;

Gao, Zhancheng ;

Xiang, Nijuan ;

Shen, Yinzhong ;

He, Zebao ;

Gu, Yong ;

Zhang, Zhiyong ;

Yang, Yi ;

Zhao, Xiang ;

Zhou, Lei ;

Li, Xiaodan ;

Zou, Shumei ;

Zhang, Ye ;

Li, Xiyan ;

Yang, Lei ;

Guo, Junfeng ;

Dong, Jie ;

Li, Qun ;

Dong, Libo ;

Zhu, Yun ;

Bai, Tian ;

Wang, Shiwen ;

Hao, Pei ;

Yang, Weizhong ;

Zhang, Yanping ;

Han, Jun ;

Yu, Hongjie ;

Li, Dexin ;

Gao, George F. ;

Wu, Guizhen ;

Wang, Yu ;

Yuan, Zhenghong ;

Shu, Yuelong .

NEW ENGLAND JOURNAL OF MEDICINE, 2013, 368 (20) :1888-1897

[2] Detection of Extensive Cross-Neutralization between Pandemic and Seasonal A/H1N1 Influenza Viruses Using a Pseudotype Neutralization Assay [J].

Labrosse, Beatrice ;

Tourdjman, Mathieu ;

Porcher, Raphael ;

LeGoff, Jerome ;

de Lamballerie, Xavier ;

Simon, Francois ;

Molina, Jean-Michel ;

Clavel, Francois .

PLOS ONE, 2010, 5 (06)

[3] Epidemiology of Human Infections with Avian Influenza A(H7N9) Virus in China [J].

Li, Qun ;

Zhou, Lei ;

Zhou, Minghao ;

Chen, Zhiping ;

Li, Furong ;

Wu, Huanyu ;

Xiang, Nijuan ;

Chen, Enfu ;

Tang, Fenyang ;

Wang, Dayan ;

Meng, Ling ;

Hong, Zhiheng ;

Tu, Wenxiao ;

Cao, Yang ;

Li, Leilei ;

Ding, Fan ;

Liu, Bo ;

Wang, Mei ;

Xie, Rongheng ;

Gao, Rongbao ;

Li, Xiaodan ;

Bai, Tian ;

Zou, Shumei ;

He, Jun ;

Hu, Jiayu ;

Xu, Yangting ;

Chai, Chengliang ;

Wang, Shiwen ;

Gao, Yongjun ;

Jin, Lianmei ;

Zhang, Yanping ;

Luo, Huiming ;

Yu, Hongjie ;

He, Jianfeng ;

Li, Qi ;

Wang, Xianjun ;

Gao, Lidong ;

Pang, Xinghuo ;

Liu, Guohua ;

Yan, Yansheng ;

Yuan, Hui ;

Shu, Yuelong ;

Yang, Weizhong ;

Wang, Yu ;

Wu, Fan ;

Uyeki, Timothy M. ;

Feng, Zijian .

NEW ENGLAND JOURNAL OF MEDICINE, 2014, 370 (06) :520-532

[4]

Molesti Eleonora, 2012, J Mol Genet Med, V7, P315

[5] Hemagglutinin pseudotyped lentiviral particles: Characterization of a new method for avian H5N1 influenza sero-diagnosis [J].

Nefkens, Isabelle ;

Garcia, Jean-Michel ;

Ling, Chu Shui ;

Lagarde, Nadege ;

Nicholls, John ;

Tang, Dong Jiang ;

Peiris, Malik ;

Buchy, Philippe ;

Altmeyer, Ralf .

JOURNAL OF CLINICAL VIROLOGY, 2007, 39 (01) :27-33

[6]

Nicoll A, 2013, EUROSURVEILLANCE, V18, P2

[7] Boosting Heterosubtypic Neutralization Antibodies in Recipients of 2009 Pandemic H1N1 Influenza Vaccine [J].

Qiu, Chao ;

Huang, Yang ;

Wang, Qian ;

Tian, Di ;

Zhang, Wanju ;

Hu, Yunwen ;

Yuan, Zhenghong ;

Zhang, Xiaoyan ;

Xu, Jianqing .

CLINICAL INFECTIOUS DISEASES, 2012, 54 (01) :17-24

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