Normalizing polydiacetylene colorimetric assays of vesicle binding across lipid systems

被引：7
作者：
Beasley, Maryssa [1 ]
Stonebraker, Alyssa R. [1 ]
Legleiter, Justin [1 ,2 ,3 ]
机构：
[1] West Virginia Univ, C Eugene Bennett Dept Chem, 217 Clark Hall, Morgantown, WV 26506 USA
[2] West Virginia Univ, WVU Rockefeller Neurosci Inst, 1 Med Ctr Dr,POB 9303, Morgantown, WV 26505 USA
[3] West Virginia Univ, Dept Neurosci, 1 Med Ctr Dr,POB 9303, Morgantown, WV 26505 USA
来源：
ANALYTICAL BIOCHEMISTRY | 2020年 / 609卷
关键词：
Membrane binding affinity; High-throughput screening; EGCG; Lipid vesicles; beta-amyloid; EGCG; MEMBRANES; GALLATE;
D O I：
10.1016/j.ab.2020.113864
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Mixed polydiacetylene (PDA) lipid vesicles mimic cell membranes and exhibit a colorimetric response induced by mechanical stress, which can be used to determine the affinity of proteins or molecules for lipid membranes. Due to a simple spectroscopic readout, PDA assays are amenable to high-throughput screens; however, these assays exhibit batch-to-batch variability. Sensitivity of the assay is also influenced by physicochemical properties associated with different lipids. Here, a method of normalizing PDA assays to reduce variability and enable direct comparison across lipid systems is described.
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页数：4
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