The role of SDF-1-CXCR4/CXCR7 axis in biological behaviors of adipose tissue-derived mesenchymal stem cells in vitro

被引：79

作者：

Li, Qiang ^{[1
]}

Zhang, Aijun ^{[1
]}

Tao, Changbo ^{[1
]}

Li, Xueyang ^{[1
]}

Jin, Peisheng ^{[1
]}

机构：

[1] Xuzhou Med Coll, Affiliated Hosp, Dept Plast Surg, Xuzhou, Jiangsu, Peoples R China

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2013年 / 441卷 / 03期

基金：

中国国家自然科学基金;

关键词：

ADSCs; SDF-1; CXCR4; CXCR7; REGENERATIVE MEDICINE; CARDIAC-FUNCTION; CXCR4; TRANSPLANTATION; ANGIOGENESIS; MOBILIZATION; MIGRATION; PROLIFERATION; REPOPULATION; RECRUITMENT;

D O I：

10.1016/j.bbrc.2013.10.071

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Numerous studies have reported that CXCR4 and CXCR7 play an essential, but differential role in stromal cell-derived factor-1 (SDF-1)-inducing cell chemotaxis, viability and paracrine actions of BMSCs. Adipose tissue-derived mesenchymal stem cells (ADSCs) have been suggested to be potential seed cells for clinical application instead of bone marrow derived stroma cell (BMSCs). However, the function of SDF-1/CXCR4 and SDF-1/CXCR7 in ADSCs is not well understood. This study was designed to analyze the effect of SDF-1/CXCR4 and SDF-1/CXCR7 axis on ADSCs biological behaviors in vitro. Using Flow cytometry and Western blot methods, we found for the first time that CXCR4/CXCR7 expression was increased after treatment with SDF-1 in ADSCs. SDF-1 promoted ADSCs paracrine, proliferation and migration abilities. CXCR4 or CXCR7 antibody suppressed ADSCs paracrine action induced by SDF-1. The migration of ADSCs can be abolished by CXCR4 antibody, while the proliferation of ADSCs was only downregulated by CXCR7 antibody. Our study indicated that the angiogenesis of ADSCs is, at least partly, mediated by SDF-1/CXCR4 and SDF-1/CXCR7 axis. However, only binding of SDF-1/CXCR7 was required for proliferation of ADSCs, and CXCR7 was required for migration of ADSCs induced by SDF-1. Our studies provide evidence that the activation of either axis may be helpful to improve the effectiveness of ADSCs-based stem cell therapy. (C) 2013 Elsevier Inc. All rights reserved.

引用

页码：675 / 680

页数：6

共 33 条

[1] IFATS Collection: Human Adipose Tissue-Derived Stem Cells Induce Angiogenesis and Nerve Sprouting Following Myocardial Infarction, in Conjunction with Potent Preservation of Cardiac Function [J].

Cai, Liying ;

Johnstone, Brian H. ;

Cook, Todd G. ;

Tan, Jian ;

Fishbein, Michael C. ;

Chen, Peng-Sheng ;

March, Keith L. .

STEM CELLS, 2009, 27 (01) :230-237

[2] Proliferation and differentiation potential of human adipose-derived mesenchymal stem cells isolated from elderly patients with osteoporotic fractures [J].

Chen, Hui-Ting ;

Lee, Mon-Juan ;

Chen, Chung-Hwan ;

Chuang, Shu-Chun ;

Chang, Li-Fu ;

Ho, Mei-Ling ;

Hung, Shao-Hung ;

Fu, Yin-Chih ;

Wang, Yan-Hsiung ;

Wang, Hsin-I ;

Wang, Gwo-Jaw ;

Kang, Lin ;

Chang, Je-Ken .

JOURNAL OF CELLULAR AND MOLECULAR MEDICINE, 2012, 16 (03) :582-592

[3] Progenitor Cell Mobilization and Recruitment: SDF-1, CXCR4, α4-integrin, and c-kit [J].

Cheng, Min ;

Qin, Gangjian .

GENETICS OF STEM CELLS, PT A, 2012, 111 :243-264

[4]

Das R, 2010, TISSUE ENG PART B-RE, V16, P159, DOI [10.1089/ten.teb.2009.0296, 10.1089/ten.TEB.2009.0296]

[5] Genetic Modification of Mesenchymal Stem Cells to Overexpress CXCR4 and CXCR7 Does Not Improve the Homing and Therapeutic Potentials of These Cells in Experimental Acute Kidney Injury [J].

Gheisari, Yousof ;

Azadmanesh, Kayhan ;

Ahmadbeigi, Naser ;

Nassiri, Seyed Mahdi ;

Golestaneh, Azadeh Fahim ;

Naderi, Mahmood ;

Vasei, Mohammad ;

Arefian, Ehsan ;

Mirab-Samiee, Siamak ;

Shafiee, Abbas ;

Soleimani, Masoud ;

Zeinali, Sirous .

STEM CELLS AND DEVELOPMENT, 2012, 21 (16) :2969-2980

[6] Treatment of Experimental Arthritis by Inducing Immune Tolerance With Human Adipose-Derived Mesenchymal Stem Cells [J].

Gonzalez, Manuel A. ;

Gonzalez-Rey, Elena ;

Rico, Laura ;

Buescher, Dirk ;

Delgado, Mario .

ARTHRITIS AND RHEUMATISM, 2009, 60 (04) :1006-1019

[7] Partial Netrin-1 Deficiency Aggravates Acute Kidney Injury [J].

Grenz, Almut ;

Dalton, Julee H. ;

Bauerle, Jessica D. ;

Badulak, Alexander ;

Ridyard, Douglas ;

Gandjeva, Aneta ;

Aherne, Carol M. ;

Brodsky, Kelley S. ;

Kim, Jae-Hwan ;

Tuder, Rubin M. ;

Eltzschig, Holger K. .

PLOS ONE, 2011, 6 (05)

[8] Cationic Liposome-Mediated CXCR4 Gene Delivery into Hematopoietic Stem/Progenitor Cells: Implications for Clinical Transplantation and Gene Therapy [J].

Gul-Uludag, Hilal ;

Xu, Peng ;

Marquez-Curtis, Leah A. ;

Xing, James ;

Janowska-Wieczorek, Anna ;

Chen, Jie .

STEM CELLS AND DEVELOPMENT, 2012, 21 (10) :1587-1596

[9] Stromal Cell-Derived Factor-1β Mediates Cell Survival through Enhancing Autophagy in Bone Marrow-Derived Mesenchymal Stem Cells [J].

Herberg, Samuel ;

Shi, Xingming ;

Johnson, Maribeth H. ;

Hamrick, Mark W. ;

Isales, Carlos M. ;

Hill, William D. .

PLOS ONE, 2013, 8 (03)

[10] SDF-1/CXCR4 mediates acute protection of cardiac function through myocardial STAT3 signaling following global ischemia/reperfusion injury [J].

Huang, Chunyan ;

Gu, Hongmei ;

Zhang, Wenjun ;

Manukyan, Mariuxi C. ;

Shou, Weinian ;

Wang, Meijing .

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY, 2011, 301 (04) :H1496-H1505

← 1 2 3 4 →