Production, mechanical properties and in vitro biocompatibility of highly aligned porous poly(ε-caprolactone) (PCL)/hydroxyapatite (HA) scaffolds

被引:24
作者
Choi, Won-Young [1 ]
Kim, Hyoun-Ee [1 ]
Koh, Young-Hag [2 ]
机构
[1] Seoul Natl Univ, Dept Sci & Engn, Seoul 151742, South Korea
[2] Korea Univ, Dept Dent Lab Sci & Engn, Seoul 136703, South Korea
关键词
Poly(epsilon-caprolactone) (PCL); Hydroxyapatite; Scaffold; Porous; Biocompatibility; COMPOSITE SCAFFOLDS; PHASE-SEPARATION; BONE; FABRICATION; ARCHITECTURE;
D O I
10.1007/s10934-012-9644-4
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
We produced highly aligned porous poly(epsilon-caprolactone) (PCL)/hydroxyapatite (HA) scaffolds by unidirectionally freezing PCL/HA solutions with various HA contents (0, 5, 10 and 20 wt% in relation to the PCL polymer) and evaluated their mechanical properties and in vitro biocompatibility to examine their potential applications in bone tissue engineering. All the prepared scaffolds had a highly aligned porous structure, in which the HA particles were uniformly dispersed in the PCL walls. The elastic modulus of the PCL/HA scaffolds significantly increased from 0.12 +/- A 0.02 to 2.65 +/- A 0.05 MPa with increasing initial HA content from 0 to 20 wt%, whereas the pore size decreased from 9.2 +/- A 0.7 to 4.2 +/- A 0.8 mu m. In addition, the PCL/HA scaffolds showed considerably enhanced in vitro cellular responses that were assessed in terms of cell attachment, proliferation and osteoblastic differentiation.
引用
收藏
页码:701 / 708
页数:8
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