Determination of dithiocarbamate fungicides by reversed-phase ion-pair liquid chromatography with chemiluminescence detection

被引：23

作者：

Nakazawa, H

Tsuda, Y

Ito, K

Yoshimura, Y

Kubo, H

Homma, H

机构：

[1] Hoshi Univ, Fac Pharmaceut Sci, Dept Analyt Chem, Shinagawa Ku, Tokyo 1428501, Japan

[2] Kitasato Univ, Dept Analyt Chem, Sagamihara, Kanagawa, Japan

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES | 2004年 / 27卷 / 04期

关键词：

antioxidants; chemiluminescence; dithiocarbamate fungicides; ion-pair chromatography; luminol; mancozeb; propineb;

D O I：

10.1081/JLC-120028258

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Dithiocarbamate fungicides react with luminol to produce an intense chemiluminescence in the presence of hexacyanoferrate(III) and hexacyanoferrate(II) in alkaline solution. Based on this fact, a simple and sensitive method for the determination of dithiocarbamate fungicides by reversed-phase ion-pair liquid chromatography with chemiluminescence was developed. The mancozeb and propineb of dithiocarbamate fungicides were able to be separated using 5 mM phosphate buffer (pH 7.5) contained 2.5 mM tetrabutylammonium hydrogen sulfate/methanol (75:25) in a short time. The proposed method could be applied to the analysis of vegetables spiked with dithiocarbamate fungicides.

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收藏

页码：705 / 713

页数：9

相关论文

共 18 条

[1] Biological monitoring of pesticide exposure: a review of analytical methods [J].

Aprea, C ;

Colosio, C ;

Mammone, T ;

Minoia, C ;

Maroni, M .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 769 (02) :191-219

[2] Determination of dithiocarbamate fungicide residues in food by a spectrophotometric method using a vertical disulfide reaction system [J].

Caldas, ED ;

Conceiçao, MH ;

Miranda, MCC ;

de Souza, LCKR ;

Lima, JF .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2001, 49 (10) :4521-4525

[3] Determination of sulfide in waters by flow-injection solid phase spectrophotometry [J].

Cassella, RJ ;

Teixeira, LSG ;

Garrigues, S ;

Costa, ACS ;

Santelli, RE ;

de la Guardia, M .

ANALYST, 2000, 125 (10) :1835-1838

[4]

DA S, 1999, J LIQ CHROMATOGR R T, V22, P463

[5] Liquid chromatography particle beam mass spectrometry determination of carbamates in lettuce and apple [J].

García-Blázquez, RM ;

Pérez-Arribas, LV ;

León-González, ME ;

Polo-Díez, LM .

JOURNAL OF LIQUID CHROMATOGRAPHY & RELATED TECHNOLOGIES, 1998, 21 (08) :1173-1183

[6] Hapten synthesis for the development of a competitive inhibition enzyme-immunoassay for thiram [J].

Gueguen, F ;

Boisdé, F ;

Queffelec, AL ;

Haelters, JP ;

Thouvenot, D ;

Corbel, B ;

Nodet, P .

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2000, 48 (10) :4492-4499

[7] DETERMINATION OF DITHIOCARBAMATES IN RESIDUES BY LIQUID-CHROMATOGRAPHY WITH SELECTIVE PRECOLUMN OR REACTION-DETECTION SYSTEMS [J].

IRTH, H ;

DEJONG, GJ ;

FREI, RW ;

BRINKMAN, UAT .

INTERNATIONAL JOURNAL OF ENVIRONMENTAL ANALYTICAL CHEMISTRY, 1990, 39 (02) :129-139

[8] GAS-LIQUID AND LIQUID-CHROMATOGRAPHIC DETERMINATION OF ZINEB AND MANEB FOR THE ASSESSMENT OF OCCUPATIONAL EXPOSURE IN THE PRODUCTION OF ORNAMENTALS [J].

JONGEN, MJM ;

RAVENSBERG, JC ;

ENGEL, R ;

LEENHEERS, LH .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 1991, 29 (07) :292-297

[9] Chemiluminescence of dithiocarbarnate fungicides based on the luminol reaction [J].

Kubo, H ;

Tsuda, Y ;

Yoshimura, Y ;

Homma, H ;

Nakazawa, H .

ANALYTICA CHIMICA ACTA, 2003, 494 (1-2) :49-53

[10] HPLC STUDY OF MANCOZEB DEGRADATION ON LEAVES [J].

LEHOTAY, J ;

KISOVA, D .

JOURNAL OF LIQUID CHROMATOGRAPHY, 1993, 16 (05) :1015-1022