Enzyme-free fluorescent biosensor for miRNA-21 detection based on MnO2 nanosheets and catalytic hairpin assembly amplification

被引：29

作者：

Ouyang, Wenjun ^{[1
]}

Liu, ZhenHua ^{[2
]}

Zhang, GuiFeng ^{[2
]}

Chen, Zheng ^{[2
]}

Guo, Longhua ^{[1
]}

Lin, Zhenyu ^{[1
]}

Qiu, Bin ^{[1
]}

Chen, Guonan ^{[1
]}

机构：

[1] Fuzhou Univ, Fujian Prov Key Lab Anal & Detect Food Safety, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Fujian, Peoples R China

[2] Fujian Med Univ, Fujian Prov Hosp, Fujian Prov Clin Coll, Dept Med Oncol, Fuzhou 350001, Peoples R China

来源：

Analytical Methods | 2016年 / 8卷 / 48期

基金：

中国国家自然科学基金;

关键词：

ROLLING CIRCLE AMPLIFICATION; HUMAN BREAST-CANCER; MICRORNA DETECTION; ISOTHERMAL AMPLIFICATION; ULTRASENSITIVE DETECTION; NANOPARTICLE PROBES; SENSING PLATFORM; GRAPHENE OXIDE; DNA; CELLS;

D O I：

10.1039/c6ay02551j

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

MicroRNAs (miRNAs) have attracted enormous interest due to their involvement in complex regulatory functions and for being closely linked to some cancers. Thus, qualitative and quantitative detection of miRNAs has become an urgent necessity. In this study, we report an enzyme-free fluorescent biosensor for miRNA-21 detection based on MnO2 nanosheets and catalytic hairpin assembly amplification. Results showed that the detection limit for miRNA-21 was 0.33 nM (S/N = 3) and the linearity range was from 1 to 50 nM. Compared with traditional methods, such strategy is convenient, rapid, and low-cost and it can have a promising potential for miRNA detection in cancer.

引用

页码：8492 / 8497

页数：6

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