Light-induced silencing of neural activity in Rosa26 knock-in mice conditionally expressing the microbial halorhodopsin eNpHR2.0

被引：8

作者：

Imayoshi, Itaru ^{[1
,2
,3
]}

Tabuchi, Sawako ^{[4
]}

Hirano, Kyoko ^{[1
,5
]}

Sakamoto, Masayuki ^{[1
,5
]}

Kitano, Satsuki ^{[1
]}

Miyachi, Hitoshi ^{[1
]}

Yamanaka, Akihiro ^{[3
,4
]}

Kageyama, Ryoichiro ^{[1
,6
]}

机构：

[1] Kyoto Univ, Inst Virus Res, Kyoto 6068507, Japan

[2] Kyoto Univ, Hakubi Ctr, Kyoto 6068507, Japan

[3] Japan Sci & Technol Agcy, Precursory Res Embryon Sci & Technol PRESTO, Kawaguchi, Saitama 3320012, Japan

[4] Natl Inst Physiol Sci, Div Cell Signaling, Okazaki, Aichi 4448787, Japan

[5] Kyoto Univ, Grad Sch Biostudies, Kyoto 6068502, Japan

[6] Japan Sci & Technol Agcy, Core Res Evolut Sci & Technol CREST, Kyoto 6068507, Japan

来源：

NEUROSCIENCE RESEARCH | 2013年 / 75卷 / 01期

基金：

日本科学技术振兴机构;

关键词：

Cre; Flp; Optogenetics; eNpHR; Rosa26; TRANSGENIC MICE; NEURONS; INTERROGATION; ACTIVATION; CIRCUITRY; GENE; VIVO;

D O I：

10.1016/j.neures.2012.03.008

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Temporally precise inhibition of genetically defined cell populations in intact nervous systems has been enabled by the microbial halorhodopsin NpHR, a fast, light-activated chloride pump. Here, we report the generation of new mouse strains that express eNpHR2-EYFP fusion proteins after Cre- and/or Flp-mediated recombination to silence neural activity in vivo. In these mouse strains, Cre/Flp recombination induced a high-level of eNpHR2-EYFP expression. Slice whole-cell patch clamp experiments confirmed that eNpHR2-EYFP-expressing neurons could be optically hyperpolarized and inhibited from firing action potentials. Thus, these mouse strains offer powerful tools for light-induced silencing of neural activity in genetically defined cell populations. (C) 2012 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.

引用

页码：53 / 58

页数：6

共 3 条

[1] Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3
Imayoshi, Itaru
Tabuchi, Sawako
Matsumoto, Mami
Kitano, Satsuki
Miyachi, Hitoshi
Kageyama, Ryoichiro
Yamanaka, Akihiro
SCIENTIFIC REPORTS, 2020, 10 (01)
[2] Light-induced silencing of neural activity in Rosa26 knock-in and BAC transgenic mice conditionally expressing the microbial halorhodopsin eNpHR3
Itaru Imayoshi
Sawako Tabuchi
Mami Matsumoto
Satsuki Kitano
Hitoshi Miyachi
Ryoichiro Kageyama
Akihiro Yamanaka
Scientific Reports, 10
[3] ROSA26 Angiotensin-Converting Enzyme 2 (ACE2) Knock-in Mice Exhibit Normal Cardiac Function But Attenuated Angiotensin II-Induced Hypertension
Bradford, Chastity N.
Pellet, Christena
Kennedy, William
Ely, Debra
Seth, Dale
Navar, L. Gabriel
Raizada, Mohan K.
Oh, S. Paul
HYPERTENSION, 2011, 58 (05) : E104 - E104

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