Analysis of 8 X-chromosomal markers in the population of central Croatia

被引:8
作者
Grskovic, Branka [1 ,2 ]
Zidkova, Anastassiya [3 ,4 ,5 ]
Stenzl, Vlastimil [6 ]
Popovic, Maja [2 ,7 ]
Primorac, Dragan [8 ,9 ,10 ,11 ,12 ]
Mrsic, Gordan [1 ,2 ]
机构
[1] Minist Interior, Gen Police Directorate, Forens Sci Ctr Ivan Vucetic, Zagreb 10000, Croatia
[2] Univ Split, Univ Ctr Forens Sci, Split, Croatia
[3] Charles Univ Prague, Fac Med 1, Inst Biol & Med Genet, Prague, Czech Republic
[4] Gen Univ Hosp Prague, Prague, Czech Republic
[5] Charles Univ Prague, Inst Forens Med & Toxycol, Fac Med 1, Prague, Czech Republic
[6] Inst Criminalist, Dept Forens Genet, Prague, Czech Republic
[7] Univ Zagreb, Fac Vet Med, Dept Biol, Zagreb 41000, Croatia
[8] Univ Split, Sch Med, Split, Croatia
[9] Univ Osijek, Sch Med, Osijek, Croatia
[10] Penn State Univ, Eberly Coll Sci, University Pk, PA 16802 USA
[11] Univ New Haven, New Haven, CT USA
[12] Genos Ltd, Zagreb, Croatia
关键词
MALE-DRIVEN EVOLUTION; LINKAGE DISEQUILIBRIUM; HUMAN GENOME; REGION; DNA; HAPLOTYPE; PURPOSES; HISTORY;
D O I
10.3325/cmj.2013.54.238
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Aim To analyze 8 X-linked short tandem repeat (STR) markers in the population of central Croatia and to evaluate their forensic efficiency. Methods We carried out a statistical analysis of the data from previously performed genetic analyses, collected during routine forensic work by the Forensic Science Centre "Ivan Vucetic." Mentype (R) Argus X-8 PCR amplification kit was used for typing the data of 99 unrelated healthy women and 78 men from central Croatia. Haplotype frequencies were calculated only in male samples. Arlequin 3.5 software was used to assess Hardy-Weinberg equilibrium (HWE), linkage disequilibrium (LD), observed and expected heterozygosity. Power of discrimination (PD) for men and women, polymorphism information content (PIC), power of exclusion, and mean exclusion chance for deficiency cases, normal trios, and duos were determined using online database ChrX-STR.org. Results In female samples, deviations from HWE (P = 0.006) for each locus were not found. LD test performed both on female and male samples revealed no significant association between markers (P = 0.002). DXS10135 was the most polymorphic locus (PIC = 0.931). PD varied from 0.692 to 0.935 in male and from 0.845 to 0.992 in female samples. Combined PD reached 99.999999% in men and 99.9999999999% in women. Conclusion Performed analyses revealed that the studied marker set contained polymorphic markers with high power of discrimination. We can conclude that Mentype (R) Argus X-8 PCR is suitable for application in the population of central Croatia. Results of this study, together with collected allele and haplotype frequencies, are the first step in establishing a national reference X-STR database based on 8 X-STR loci.
引用
收藏
页码:238 / 247
页数:10
相关论文
共 50 条
  • [31] Population genetic investigation of eight X-chromosomal short tandem repeat loci from a northeast German sample
    Tetzlaff, Sylvio
    Wegener, Rudolf
    Lindner, Iris
    FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2012, 6 (06) : E155 - E156
  • [32] Forensic efficiency and genetic polymorphisms of 12 X-chromosomal STR loci in Northeastern Thai populations
    Srithawong, Suparat
    Muisuk, Kanha
    Prakhun, Nonglak
    Tungpairojwong, Nisarat
    Kutanan, Wibhu
    MOLECULAR GENETICS AND GENOMICS, 2024, 299 (01)
  • [33] A simple method for calculating the likelihood ratio in a kinship test using X-chromosomal markers incorporating linkage, linkage disequilibrium, and mutation
    Fukuta, Mamiko
    Gaballah, Mohammed Hassan
    Kato, Hideaki
    Aoki, Yasuhiro
    LEGAL MEDICINE, 2018, 32 : 9 - 18
  • [34] Population genetics, diversity, forensic characteristics of four Chinese populations inferred from X-chromosomal short tandem repeats
    He, Guanglin
    Zou, Xing
    Wang, Mengge
    Liu, Jing
    Wang, Fei
    Hou, Yiping
    Wang, Zheng
    LEGAL MEDICINE, 2020, 43
  • [35] FamLinkX - implementation of a general model for likelihood computations for X-chromosomal marker data
    Kling, Daniel
    Dell'Amico, Barbara
    Tillmar, Andreas O.
    FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2015, 17 : 1 - 7
  • [36] Development of pentaplex PCR and genetic analysis of X chromosomal STRs in Punjabi population of Pakistan
    Nadeem, Asif
    Babar, Masroor Ellahi
    Hussain, Manzoor
    Tahir, Mohammad A.
    MOLECULAR BIOLOGY REPORTS, 2009, 36 (07) : 1671 - 1675
  • [37] Analysis of 12 X-STR loci in the population of south Croatia
    Mrsic, Gordan
    Ozretic, Petar
    Crnjac, Josip
    Merkas, Sinisa
    Racic, Ivana
    Rozic, Sara
    Sukser, Viktorija
    Popovic, Maja
    Korolija, Marina
    MOLECULAR BIOLOGY REPORTS, 2017, 44 (01) : 183 - 189
  • [38] Application of a novel multiplex polymerase chain reaction system for 12 X-chromosomal short tandem repeats to a Japanese population study
    Nishi, Takeki
    Kurosu, Akira
    Sugano, Yukiko
    Kaminiwa, Junko
    Sekine, Yuko
    Yano, Shizue
    Honda, Katsuya
    LEGAL MEDICINE, 2013, 15 (01) : 43 - 46
  • [39] Analysis of four novel X-chromosomal short tandem repeats within 71 kb of the Xp22.3 region
    Nishi, Takeki
    Fukui, Kenji
    Iwadate, Kimiharu
    INTERNATIONAL JOURNAL OF LEGAL MEDICINE, 2017, 131 (05) : 1229 - 1233
  • [40] X-Chromosomal Genetic Diversity and Linkage Disequilibrium Patterns in Amerindians and Non-Amerindian Populations
    Amorim, Carlos Eduardo G.
    Wang, Sijia
    Marrero, Andrea R.
    Salzano, Francisco M.
    Ruiz-Linares, Andres
    Bortolini, Maria Catira
    AMERICAN JOURNAL OF HUMAN BIOLOGY, 2011, 23 (03) : 299 - 304