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Analysis of 8 X-chromosomal markers in the population of central Croatia
被引:8
作者:
Grskovic, Branka
[1
,2
]
Zidkova, Anastassiya
[3
,4
,5
]
Stenzl, Vlastimil
[6
]
Popovic, Maja
[2
,7
]
Primorac, Dragan
[8
,9
,10
,11
,12
]
Mrsic, Gordan
[1
,2
]
机构:
[1] Minist Interior, Gen Police Directorate, Forens Sci Ctr Ivan Vucetic, Zagreb 10000, Croatia
[2] Univ Split, Univ Ctr Forens Sci, Split, Croatia
[3] Charles Univ Prague, Fac Med 1, Inst Biol & Med Genet, Prague, Czech Republic
[4] Gen Univ Hosp Prague, Prague, Czech Republic
[5] Charles Univ Prague, Inst Forens Med & Toxycol, Fac Med 1, Prague, Czech Republic
[6] Inst Criminalist, Dept Forens Genet, Prague, Czech Republic
[7] Univ Zagreb, Fac Vet Med, Dept Biol, Zagreb 41000, Croatia
[8] Univ Split, Sch Med, Split, Croatia
[9] Univ Osijek, Sch Med, Osijek, Croatia
[10] Penn State Univ, Eberly Coll Sci, University Pk, PA 16802 USA
[11] Univ New Haven, New Haven, CT USA
[12] Genos Ltd, Zagreb, Croatia
关键词:
MALE-DRIVEN EVOLUTION;
LINKAGE DISEQUILIBRIUM;
HUMAN GENOME;
REGION;
DNA;
HAPLOTYPE;
PURPOSES;
HISTORY;
D O I:
10.3325/cmj.2013.54.238
中图分类号:
R5 [内科学];
学科分类号:
1002 ;
100201 ;
摘要:
Aim To analyze 8 X-linked short tandem repeat (STR) markers in the population of central Croatia and to evaluate their forensic efficiency. Methods We carried out a statistical analysis of the data from previously performed genetic analyses, collected during routine forensic work by the Forensic Science Centre "Ivan Vucetic." Mentype (R) Argus X-8 PCR amplification kit was used for typing the data of 99 unrelated healthy women and 78 men from central Croatia. Haplotype frequencies were calculated only in male samples. Arlequin 3.5 software was used to assess Hardy-Weinberg equilibrium (HWE), linkage disequilibrium (LD), observed and expected heterozygosity. Power of discrimination (PD) for men and women, polymorphism information content (PIC), power of exclusion, and mean exclusion chance for deficiency cases, normal trios, and duos were determined using online database ChrX-STR.org. Results In female samples, deviations from HWE (P = 0.006) for each locus were not found. LD test performed both on female and male samples revealed no significant association between markers (P = 0.002). DXS10135 was the most polymorphic locus (PIC = 0.931). PD varied from 0.692 to 0.935 in male and from 0.845 to 0.992 in female samples. Combined PD reached 99.999999% in men and 99.9999999999% in women. Conclusion Performed analyses revealed that the studied marker set contained polymorphic markers with high power of discrimination. We can conclude that Mentype (R) Argus X-8 PCR is suitable for application in the population of central Croatia. Results of this study, together with collected allele and haplotype frequencies, are the first step in establishing a national reference X-STR database based on 8 X-STR loci.
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页码:238 / 247
页数:10
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