Resistance of Bovine Spongiform Encephalopathy (BSE) Prions to Inactivation

被引:74
作者
Giles, Kurt [1 ,2 ]
Glidden, David V. [3 ]
Beckwith, Robyn [1 ]
Seoanes, Rose [1 ]
Peretz, David [1 ,2 ]
DeArmond, Stephen J. [1 ,4 ]
Prusiner, Stanley B. [1 ,2 ,5 ]
机构
[1] Univ Calif San Francisco, Inst Neurodegenerat Dis, San Francisco, CA 94143 USA
[2] Univ Calif San Francisco, Dept Neurol, San Francisco, CA 94143 USA
[3] Univ Calif San Francisco, Dept Epidemiol & Biostat, San Francisco, CA 94143 USA
[4] Univ Calif San Francisco, Dept Pathol, San Francisco, CA USA
[5] Univ Calif San Francisco, Dept Biochem & Biophys, San Francisco, CA 94143 USA
基金
美国国家卫生研究院;
关键词
D O I
10.1371/journal.ppat.1000206
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Distinct prion strains often exhibit different incubation periods and patterns of neuropathological lesions. Strain characteristics are generally retained upon intraspecies transmission, but may change on transmission to another species. We investigated the inactivation of two related prions strains: BSE prions from cattle and mouse-passaged BSE prions, termed 301V. Inactivation was manipulated by exposure to sodium dodecyl sulfate (SDS), variations in pH, and different temperatures. Infectivity was measured using transgenic mouse lines that are highly susceptible to either BSE or 301V prions. Bioassays demonstrated that BSE prions are up to 1,000-fold more resistant to inactivation than 301V prions while Western immunoblotting showed that short acidic SDS treatments reduced protease-resistant PrP(Sc) from BSE prions and 301V prions at similar rates. Our findings argue that despite being derived from BSE prions, mouse 301V prions are not necessarily a reliable model for cattle BSE prions. Extending these comparisons to human sporadic Creutzfeldt- Jakob disease and hamster Sc237 prions, we found that BSE prions were 10- and 10(6)-fold more resistant to inactivation, respectively. Our studies contend that any prion inactivation procedures must be validated by bioassay against the prion strain for which they are intended to be used.
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页数:9
相关论文
共 62 条
[1]   DOES AGENT OF SCRAPIE REPLICATE WITHOUT NUCLEIC ACID [J].
ALPER, T ;
CRAMP, WA ;
HAIG, DA ;
CLARKE, MC .
NATURE, 1967, 214 (5090) :764-&
[2]   EXCEPTIONALLY SMALL SIZE OF SCRAPIE AGENT [J].
ALPER, T ;
HAIG, DA ;
CLARKE, MC .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1966, 22 (03) :278-&
[3]  
[Anonymous], MODELING SURVIVAL DA
[4]   BSE priors propagate as either variant CJD-like or sporadic CJD-like prion strains in transgenic mice expressing human prion protein [J].
Asante, EA ;
Linehan, JM ;
Desbruslais, M ;
Joiner, S ;
Gowland, I ;
Wood, AL ;
Welch, J ;
Hill, AF ;
Lloyd, SE ;
Wadsworth, JDF ;
Collinge, J .
EMBO JOURNAL, 2002, 21 (23) :6358-6366
[5]   DISTINCT PRP PROPERTIES SUGGEST THE MOLECULAR-BASIS OF STRAIN VARIATION IN TRANSMISSIBLE MINK ENCEPHALOPATHY [J].
BESSEN, RA ;
MARSH, RF .
JOURNAL OF VIROLOGY, 1994, 68 (12) :7859-7868
[6]   A CONFIDENCE-INTERVAL FOR THE MEDIAN SURVIVAL-TIME [J].
BROOKMEYER, R ;
CROWLEY, J .
BIOMETRICS, 1982, 38 (01) :29-41
[7]   CHEMICAL DISINFECTION OF CREUTZFELDT-JAKOB DISEASE VIRUS [J].
BROWN, P ;
GIBBS, CJ ;
AMYX, HL ;
KINGSBURY, DT ;
ROHWER, RG ;
SULIMA, MP ;
GAJDUSEK, DC .
NEW ENGLAND JOURNAL OF MEDICINE, 1982, 306 (21) :1279-1282
[8]   Effects on instruments of the World Health Organization - Recommended protocols for decontamination after possible exposure to transmissible spongiform encephalopathy-contaminated tissue [J].
Brown, SA ;
Merritt, K ;
Woods, TO ;
Busick, DN .
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 2005, 72B (01) :186-190
[9]   TRANSMISSION OF BOVINE SPONGIFORM ENCEPHALOPATHY AND SCRAPIE TO MICE - STRAIN VARIATION AND THE SPECIES BARRIER [J].
BRUCE, M ;
CHREE, A ;
MCCONNELL, I ;
FOSTER, J ;
PEARSON, G ;
FRASER, H .
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 1994, 343 (1306) :405-411
[10]   BIOLOGICAL EVIDENCE THAT SCRAPIE AGENT HAS AN INDEPENDENT GENOME [J].
BRUCE, ME ;
DICKINSON, AG .
JOURNAL OF GENERAL VIROLOGY, 1987, 68 :79-89