Comprehensive transcriptome analysis and flavonoid profiling of Ginkgo leaves reveals flavonoid content alterations in day-night cycles

被引：30

作者：

Ni, Jun ^{[1
,2
]}

Dong, Lixiang ^{[1
,2
]}

Jiang, Zhifang ^{[1
,2
]}

Yang, Xiuli ^{[1
,2
]}

Chen, Ziying ^{[1
]}

Wu, Yuhuan ^{[1
]}

Xu, Maojun ^{[1
,2
]}

机构：

[1] Hangzhou Normal Univ, Coll Life & Environm Sci, Key Lab Hangzhou City Qual & Safety Agr Prod, Hangzhou, Zhejiang, Peoples R China

[2] Hangzhou Normal Univ, Zhejiang Prov Key Lab Genet Improvement & Qual Co, Hangzhou, Zhejiang, Peoples R China

来源：

PLOS ONE | 2018年 / 13卷 / 03期

基金：

中国国家自然科学基金;

关键词：

CIRCADIAN CLOCKS; RNA-SEQ; BILOBA; BIOSYNTHESIS; GENE; EXPRESSION; GROWTH; ACCUMULATION; ALIGNMENT; EXTRACT;

D O I：

10.1371/journal.pone.0193897

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ginkgo leaves are raw materials for flavonoid extraction. Thus, the timing of their harvest is important to optimize the extraction efficiency, which benefits the pharmaceutical industry. In this research, we compared the transcriptomes of Ginkgo leaves harvested at midday and midnight. The differentially expressed genes with the highest probabilities in each step of flavonoid biosynthesis were down-regulated at midnight. Furthermore, real-time PCR corroborated the transcriptome results, indicating the decrease in flavonoid biosynthesis at midnight. The flavonoid profiles of Ginkgo leaves harvested at midday and midnight were compared, and the total flavonoid content decreased at midnight. A detailed analysis of individual flavonoids showed that most of their contents were decreased by various degrees. Our results indicated that circadian rhythms affected the flavonoid contents in Ginkgo leaves, which provides valuable information for optimizing their harvesting times to benefit the pharmaceutical industry.

引用

页数：17

共 54 条

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