Novel Conformation of an RNA Structural Switch

被引：16

作者：

Kennedy, Scott D. ^{[2
]}

Kierzek, Ryszard ^{[3
]}

Turner, Douglas H. ^{[1
]}

机构：

[1] Univ Rochester, Dept Chem, Rochester, NY 14627 USA

[2] Univ Rochester, Sch Med & Dent, Dept Biochem & Biophys, Rochester, NY 14642 USA

[3] Polish Acad Sci, Inst Bioorgan Chem, Poznan, Poland

来源：

BIOCHEMISTRY | 2012年 / 51卷 / 46期

基金：

美国国家卫生研究院;

关键词：

PURINE NUCLEOSIDES; BASE-PAIRS; STACKING; PREDICTION; LOOP;

D O I：

10.1021/bi301372t

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The RNA duplex, (5'GACGAGUGUCA)(2), has two conformations in equilibrium. The nuclear magnetic resonance solution structure reveals that the major conformation of the loop, 5'GAGU/3'UGAG, is novel and contains two unusual Watson-Crick/Hoogsteen GG pairs with G residues in the syn conformation, two A residues stacked on each other in the center of the helix with inverted sugars, and two bulged-out U residues. The structure provides a benchmark for testing approaches for predicting local RNA structure and a sequence that allows the design of a unique arrangement of functional groups and/or a conformational switch into nucleic acids.

引用

页码：9257 / 9259

页数：3

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