Somatic embryogenesis and mass spectrometric identification of proteins related to somatic embryogenesis in Eruca sativa

被引：9

作者：

Chen, Kan ^{[1
]}

Wu, Hai-Jun ^{[1
]}

Chen, Jian-Feng ^{[1
]}

Cheng, Xiao-Fang ^{[1
]}

Jing, Xiao ^{[1
]}

Wang, Xin-Yu ^{[1
]}

机构：

[1] Lanzhou Univ, Inst Cell Biol, Sch Life Sci, Lanzhou 730000, Peoples R China

来源：

PLANT BIOTECHNOLOGY REPORTS | 2012年 / 6卷 / 02期

关键词：

Somatic embryogenesis; Mass spectrometry; Sucrose synthase; Phospholipase D; Enolase; Eruca sativa; HYPOCOTYL EXPLANTS; PROTEOMIC ANALYSIS; HIGHER-PLANTS; PICEA-GLAUCA; SUCROSE; EXPRESSION; ENOLASE; EMBRYOS; SPRUCE; METABOLISM;

D O I：

10.1007/s11816-011-0203-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Several different proteins expressed in embryogenic and nonembryogenic Eruca sativa calli were identified by combining one-dimensional SDS-PAGE protein mapping with matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis. By querying the widely recognized MASCOT search engine, it was found that three of the proteins that were particularly strongly expressed in the embryogenic callus represented sucrose synthase, phospholipase D, and enolase, respectively. RT-PCR analysis also confirmed that the gene coding for enolase was transcribed especially strongly in the embryogenic callus but not in nonembyogenic callus. Finally, the relationship between the three proteins and somatic embryogenesis is discussed.

引用

页码：113 / 122

页数：10

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