Staining and embedding the whole mouse brain for electron microscopy

被引：2

作者：

Mikula, Shawn ^{[1
]}

Binding, Jonas ^{[1
]}

Denk, Winfried ^{[1
]}

机构：

[1] Max Planck Inst Med Res, Heidelberg, Germany

来源：

NATURE METHODS | 2012年 / 9卷 / 12期

关键词：

OSMIUM TETROXIDE; CONTRAST; PENETRATION; FIXATION; RATES; OTO;

D O I：

10.1038/NMETH.2213

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of methods for imaging large contiguous volumes with the electron microscope could allow the complete mapping of a whole mouse brain at the single-axon level. We developed a method based on prolonged immersion that enables staining and embedding of the entire mouse brain with uniform myelin staining and a moderate preservation of the tissue's ultrastructure. We tested the ability to follow myelinated axons using serial block-face electron microscopy.

引用

页码：1198 / U96

页数：6

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