Analytical application of polymethylene blue-multiwalled carbon nanotubes modified glassy carbon electrode on anticancer drug irinotecan and determination of its ionization constant value

被引:35
作者
Karadas, Nurgul [1 ,2 ]
Sanli, Senem [3 ]
Akmese, Bediha [2 ]
Dogan-Topal, Burcu [1 ]
Can, Alp [4 ]
Ozkan, Sibel A. [1 ]
机构
[1] Ankara Univ, Dept Analyt Chem, Fac Pharm, TR-06100 Ankara, Turkey
[2] Hitit Univ, Dept Chem, Fac Sci & Arts, Corum, Turkey
[3] Usak Univ, Dept Chem, Fac Sci & Arts, Usak, Turkey
[4] Ankara Univ, Fac Med, Dept Histol & Embryol, TR-06100 Ankara, Turkey
关键词
Carbon nanotubes; Irinotecan; Poly (methylene blue); Ionization constant; Reversed-phase liquid chromatography; PERFORMANCE LIQUID-CHROMATOGRAPHY; CARBOXYLATE FORMS; METABOLITE SN-38; PK(A) VALUES; PLASMA; CPT-11; LACTONE; POLYMER; SENSOR; MEDIA;
D O I
10.1016/j.talanta.2013.07.006
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
The voltammetric behavior of anticancer drug irinotecan (IRT) was investigated at poly (methylene blue)/multi-walled carbon nanotube (PMB/MWCNT) modified glassy carbon electrode (GCE). The modified electrode surface was characterized by a scanning electron microscope (SEM). The PMB/MWCNT modified GCE exhibits a distinct shift of the oxidation potential of IRT on the cathodic direction and a considerable enhancement of the peak current compared with bare electrode. The calibration curve was linear between the concentration range 8.0 x 10(-6) and 8.0 x 10(-5) M with the detection limit of 2.14 x 10(-7) M by differential pulse voltammetry in pH 10.0 Britton-Robinson buffer solution. Controlled potential coulometry was applied to find transferred electron numbers due to the oxidation of IRT. In this study, the pK(a) value of IRT was also determined by the dependence of the retention factor on the pH of the mobile phase. The effect of the mobile phase composition on the ionization constant was studied by measuring the pK(a) at different acetonitrile-water mixtures, ranging between 35 and 50% (v/v) using the reversed-phase liquid chromatography (RP-LC) method with UV detector. IRT was exposed to thermal, photolytic, hydrolytic and oxidative stress conditions, and the stressed samples were detected by the proposed method. Sensitive, rapid, and fully validated electrochemical and RP-LC methods for the determination of IRT in its dosage form were presented in details. (C) 2013 Elsevier B.V. All rights reserved.
引用
收藏
页码:911 / 919
页数:9
相关论文
共 50 条
[1]   Electrochemical study of nisoldipine: analytical application in pharmaceutical forms and photodegradation [J].
Alvarez-Lueje, A ;
Naranjo, L ;
Nunez-Vergara, LJ ;
Squella, JA .
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1998, 16 (05) :853-862
[2]   CATALYSIS OF ELECTROCHEMICAL REACTIONS AT REDOX POLYMER COATED ELECTRODES - MEDIATION OF THE FE(III)/FE(II) OXIDOREDUCTION BY A POLYVINYLPYRIDINE POLYMER CONTAINING COORDINATIVELY ATTACHED BISBIPYRIDINE CHLORORUTHENIUM REDOX CENTERS [J].
ANDRIEUX, CP ;
HAAS, O ;
SAVEANT, JM .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (26) :8175-8182
[3]  
[Anonymous], 2002, ACD PKA DB VERS 6 09
[4]  
[Anonymous], 1962, ELECTROLYTES
[5]  
Balaram V.M., 2007, E-J CHEM, V4, P128
[6]   Carbon nanotube actuators [J].
Baughman, RH ;
Cui, CX ;
Zakhidov, AA ;
Iqbal, Z ;
Barisci, JN ;
Spinks, GM ;
Wallace, GG ;
Mazzoldi, A ;
De Rossi, D ;
Rinzler, AG ;
Jaschinski, O ;
Roth, S ;
Kertesz, M .
SCIENCE, 1999, 284 (5418) :1340-1344
[7]   Simple liquid chromatography method for the quantification of irinotecan and SN38 in sheep plasma: Application to in vivo pharmacokinetics after pulmonary artery chemoembolization using drug eluting beads [J].
Baylatry, Minh-Tam ;
Joly, Anne-Christine ;
Pelage, Jean-Pierre ;
Bengrine-Lefevre, Leila ;
Prugnaud, Jean-Louis ;
Laurent, Alex ;
Fernandez, Christine .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2010, 878 (9-10) :738-742
[8]   Glucose sensitivity of platinum-based alloys incorporated in polypyrrole films at neutral media [J].
Becerik, I ;
Kadirgan, F .
SYNTHETIC METALS, 2001, 124 (2-3) :379-384
[9]  
Berry I.R., 2001, Validation of active pharmaceutical ingredients
[10]   Simultaneous determination of the lactone and carboxylate forms of the camptothecin derivative CPT-11 and its metabolite SN-38 in plasma by high-performance liquid chromatography [J].
Chollet, DF ;
Goumaz, L ;
Renard, A ;
Montay, G ;
Vernillet, L ;
Arnera, V ;
Mazzo, DJ .
JOURNAL OF CHROMATOGRAPHY B, 1998, 718 (01) :163-175