A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases

被引：23

作者：

Lim, Shimin ^{[1
]}

Wang, Yin ^{[2
,3
]}

Yu, Xueyao ^{[4
]}

Huang, Yian ^{[5
]}

Featherstone, Mark S. ^{[1
]}

Sampath, Karuna ^{[1
,2
,3
]}

机构：

[1] Nanyang Technol Univ, Sch Biol Sci, Singapore 639798, Singapore

[2] Natl Univ Singapore, Temasek Life Sci Lab, Singapore 117604, Singapore

[3] Natl Univ Singapore, Dept Biol Sci, Singapore 117543, Singapore

[4] Temasek Polytech, Sch Appl Sci, Singapore 529757, Singapore

[5] NUS High Sch Math & Sci, Singapore 129957, Singapore

来源：

GENOME BIOLOGY | 2013年 / 14卷 / 07期

关键词：

ZINC-FINGER NUCLEASES; HUMAN GENOME; FLOOR PLATE; TALEN; SYSTEM; REARRANGEMENTS; MUTATION; REVEALS; GENES; RNA;

D O I：

10.1186/gb-2013-14-7-r69

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Precise and effective genome-editing tools are essential for functional genomics and gene therapy. Targeting nucleases have been successfully used to edit genomes. However, whole-locus or element-specific deletions abolishing transcript expression have not previously been reported. Here, we show heritable targeting of locus-specific deletions in the zebrafish nodal-related genes squint (sqt) and cyclops (cyc). Our strategy of heritable chromosomal editing can be used for disease modeling, analyzing gene clusters, regulatory regions, and determining the functions of non-coding RNAs in genomes.

引用

页数：11

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