Identification of Genomic Variants of SARS-CoV-2 Using Nanopore Sequencing

被引：5

作者：

Capraru, Ionut Dragos ^{[1
,2
,3
]}

Romanescu, Mirabela ^{[2
,4
,5
]}

Anghel, Flavia Medana ^{[2
,4
]}

Oancea, Cristian ^{[6
]}

Marian, Catalin ^{[4
,5
]}

Sirbu, Ioan Ovidiu ^{[4
,5
]}

Chis, Aimee Rodica ^{[4
,5
]}

Ciordas, Paula Diana ^{[2
,4
,5
]}

机构：

[1] Victor Babes Univ Med & Pharm, Discipline Epidemiol, Timisoara 300041, Romania

[2] Victor Babes Univ Med & Pharm, Doctoral Sch, Timisoara 300041, Romania

[3] Publ Hlth Author Timis Cty, Timisoara 300029, Romania

[4] Victor Babes Univ Med & Pharm, Discipline Biochem, Timisoara 300041, Romania

[5] Victor Babes Univ Med & Pharm, Ctr Complex Network Sci, Timisoara 300041, Romania

[6] Victor Babes Univ Med & Pharm, Discipline Pulmonol, Timisoara 300041, Romania

来源：

MEDICINA-LITHUANIA | 2022年 / 58卷 / 12期

关键词：

SARS-CoV-2; sequencing; Nanopore; MinION; Ion Torrent; VIRUS; ZIKA;

D O I：

10.3390/medicina58121841

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Background and Objectives: SARS-CoV-2 is the first global threat and life-changing event of the twenty-first century. Although efficient treatments and vaccines have been developed, due to the virus's ability to mutate in key regions of the genome, whole viral genome sequencing is needed for efficient monitoring, evaluation of the spread, and even the adjustment of the molecular diagnostic assays. Materials and Methods: In this study, Nanopore and Ion Torrent sequencing technologies were used to detect the main SARS-CoV-2 circulating strains in Timis County, Romania, between February 2021 and May 2022. Results: We identified 22 virus lineages belonging to seven clades: 20A, 20I (Alpha, V1), 21B (Kappa), 21I (Delta), 21J (Delta), 21K (Omicron), and 21L (Omicron). Conclusions: Results obtained with both methods are comparable, and we confirm the utility of Nanopore sequencing in large-scale epidemiological surveillance due to the lower cost and reduced time for library preparation.

引用

页数：12

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