Two Highly Pathogenic Avian Influenza H5N1 Viruses of Clade 2.3.2.1 with Similar Genetic Background but with Different Pathogenicity in Mice and Ducks

被引：23

作者：

Hu, J. ^{[1
]}

Zhao, K. ^{[1
]}

Liu, X. ^{[1
]}

Wang, X. ^{[1
]}

Chen, Z. ^{[1
]}

Liu, X. ^{[1
]}

机构：

[1] Yangzhou Univ, Sch Vet Med, Anim Infect Dis Lab, Yangzhou 225009, Jiangsu, Peoples R China

来源：

TRANSBOUNDARY AND EMERGING DISEASES | 2013年 / 60卷 / 02期

关键词：

avian influenza; HPAIV; H5N1; genetic background; pathogenicity; animal models; INNATE IMMUNE-RESPONSES; SINGLE-AMINO-ACID; A VIRUSES; HONG-KONG; CYTOKINE RESPONSES; VIRAL POLYMERASE; MOLECULAR-BASIS; HIGH VIRULENCE; MOUSE MODEL; HUMAN-CELLS;

D O I：

10.1111/j.1865-1682.2012.01325.x

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

A number of genetic markers for virulence of avian influenza viruses (AIVs) in different hosts have been identified. However, we isolated two H5N1 AIVs, A/Chicken/Jiangsu/k0402/2010(CK/10) and A/Goose/Jiangsu/k0403/2010(GS/10) with similar genetic background, but most well-defined molecular markers for virulence in mammals and avian species were not found in both viral genomes. In addition, pathogenicity of this pair of viruses in different hosts remains unclear. Therefore, we evaluated their pathogenicity in chickens, mice, ducks and guinea pigs. Infection of CK/10 and GS/10 in chickens caused 100% mortality within 24h. Mouse experiment showed that CK/10 was highly pathogenic (MLD50=0.33 log10 EID50), whereas GS/10 was avirulent (MLD50>6.32 log10 EID50). Interestingly, the virulence of CK/10 in ducks (DLD50=3.83 log10 EID50) was higher than that of GS/10 (DLD50=7.7 log10 EID50), which correlated with viral pathogenicity in mice. Although CK/10 and GS/10 showed distinct pathogenicity in mice, they both were lethal to guinea pigs, with CK/10 replicating to higher titres in airways than GS/10. Collectively, these findings suggest that AIVs with similar genetic backgrounds may exhibit distinct pathogenicity in specific hosts and that some unknown molecular markers for virulence may exist and need to be identified.

引用

页码：127 / 139

页数：13

共 58 条

[1]

[Anonymous], 2011, FAO OIE WHO TECHN UP

[2] Early and sustained innate immune response defines pathology and death in nonhuman primates infected by highly pathogenic influenza virus [J].

Baskin, Carole R. ;

Bielefeldt-Ohmann, Helle ;

Tumpey, Terrence M. ;

Sabourin, Patrick J. ;

Long, James P. ;

Garcia-Sastre, Adolfo ;

Tolnay, Airn-E. ;

Albrecht, Randy ;

Pyles, John A. ;

Olson, Pam H. ;

Aicher, Lauri D. ;

Rosenzweig, Elizabeth R. ;

Murali-Krishna, Kaja ;

Clark, Edward A. ;

Kotur, Mark S. ;

Fornek, Jamie L. ;

Proll, Sean ;

Palermo, Robert E. ;

Sabourin, Carol L. ;

Katze, Michael G. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (09) :3455-3460

[3] Gene Expression Analysis of Host Innate Immune Responses during Lethal H5N1 Infection in Ferrets [J].

Cameron, Cheryl M. ;

Cameron, Mark J. ;

Bermejo-Martin, Jesus F. ;

Ran, Longsi ;

Xu, Luoling ;

Turner, Patricia V. ;

Ran, Ran ;

Danesh, Ali ;

Fang, Yuan ;

Chan, Pak-Kei M. ;

Mytle, Nutan ;

Sullivan, Timothy J. ;

Collins, Tassie L. ;

Johnson, Michael G. ;

Medina, Julio C. ;

Rowe, Thomas ;

Kelvin, David J. .

JOURNAL OF VIROLOGY, 2008, 82 (22) :11308-11317

[4] Lethal Dissemination of H5N1 Influenza Virus Is Associated with Dysregulation of Inflammation and Lipoxin Signaling in a Mouse Model of Infection [J].

Cilloniz, Cristian ;

Pantin-Jackwood, Mary J. ;

Ni, Chester ;

Goodman, Alan G. ;

Peng, Xinxia ;

Proll, Sean C. ;

Carter, Victoria S. ;

Rosenzweig, Elizabeth R. ;

Szretter, Kristy J. ;

Katz, Jacqueline M. ;

Korth, Marcus J. ;

Swayne, David E. ;

Tumpey, Terrence M. ;

Katze, Michael G. .

JOURNAL OF VIROLOGY, 2010, 84 (15) :7613-7624

[5] Human influenza A H5N1 virus related to a highly pathogenic avian influenza virus [J].

Claas, ECJ ;

Osterhaus, ADME ;

van Beek, R ;

De Jong, JC ;

Rimmelzwaan, GF ;

Senne, DA ;

Krauss, S ;

Shortridge, KF ;

Webster, RG .

LANCET, 1998, 351 (9101) :472-477

[6] Crystal structures of oseltamivir-resistant influenza virus neuraminidase mutants [J].

Collins, Patrick J. ;

Haire, Lesley F. ;

Lin, Yi Pu ;

Liu, Junfeng ;

Russell, Rupert J. ;

Walker, Philip A. ;

Skehel, John J. ;

Martin, Stephen R. ;

Hay, Alan J. ;

Gamblin, Steven J. .

NATURE, 2008, 453 (7199) :1258-U61

[7] A single mutation in the PB1-F2 of H5N1 (HK/97) and 1918 influenza A viruses contributes to increased virulence [J].

Conenello, Gina M. ;

Zamarin, Dmitriy ;

Perrone, Lucy A. ;

Tumpey, Terrence ;

Palese, Peter .

PLOS PATHOGENS, 2007, 3 (10) :1414-1421

[8] THE NEURAMINIDASES OF THE VIRULENT AND AVIRULENT-A/CHICKEN PENNSYLVANIA 83 (H5N2) INFLUENZA-A VIRUSES - SEQUENCE AND ANTIGENIC ANALYSES [J].

DESHPANDE, KL ;

NAEVE, CW ;

WEBSTER, RG .

VIROLOGY, 1985, 147 (01) :49-60

[9] Two amino acid residues in the matrix protein M1 contribute to the virulence difference of H5N1 avian influenza viruses in mice [J].

Fan, Shufang ;

Deng, Guohua ;

Song, Jiasheng ;

Tian, Guobin ;

Suo, Yongbing ;

Jiang, Yongping ;

Guan, Yuntao ;

Bu, Zhigao ;

Kawaola, Yoshihiro ;

Chen, Hualan .

VIROLOGY, 2009, 384 (01) :28-32

[10] A Single-Amino-Acid Substitution in a Polymerase Protein of an H5N1 Influenza Virus Is Associated with Systemic Infection and Impaired T-Cell Activation in Mice [J].

Fornek, Jamie L. ;

Gillim-Ross, Laura ;

Santos, Celia ;

Carter, Victoria ;

Ward, Jerrold M. ;

Cheng, Lily I. ;

Proll, Sean ;

Katze, Michael G. ;

Subbarao, Kanta .

JOURNAL OF VIROLOGY, 2009, 83 (21) :11102-11115

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