Purpose. This in vitro study compared the ability of several recently introduced l-bottle adhesives to theirpreceding multiple-step dentin bonding agents in reducing microleakage around Class V composite restorations. Material and methods. Facial and lingual Class V cavities were prepared in 48 molar teeth, which were randomly divided into 6 equal groups. Three groups used the fourth-generation multiple-step systems, and 3 used the fifth-generation single-borde adhesives. ALL cavities were restored by composite and subjected to thermocycling and intermittent occlusal loading. After immersion in 0.5% basic fuchsin, the teeth were cut faciolingually in 5 consecutive sections and evaluated for dye penetration using a binocular stereomicroscope. Results. There was no statistically significant difference in the degree of microleakage comparing the adhesive's generation and manufacturer and the location of the cavity margins. A significant interaction was found between margin location and manufacturer, which implied that the sealing capacity at the enamel and cementum margins was material-specific. Conclusion. Scotchbond Multi-Purpose and Single Bond adhesives provided the best seal for enamel margins, and One-Step and Optibond FL adhesives were best for cementum margins.