Expression analysis of mir-17-5p, mir-20a and let-7a microRNAs and their target proteins in CD34+ bone marrow cells of patients with myelodysplastic syndromes

被引：30

作者：

Vasilatou, Diamantina ^{[1
,2
]}

Papageorgiou, Sotirios G. ^{[1
,2
]}

Kontsioti, Frieda ^{[1
,2
]}

Kontos, Christos K. ^{[3
,4
]}

Tsiotra, Panayiota ^{[1
,2
]}

Mpakou, Vassiliki ^{[1
,2
]}

Pavlou, Maria-Angeliki S. ^{[4
]}

Economopoulou, Christina ^{[1
,2
]}

Dimitriadis, George ^{[1
,2
]}

Dervenoulas, John ^{[1
,2
]}

Pappa, Vasiliki ^{[1
,2
]}

机构：

[1] Univ Athens, Sch Med, Attikon Univ Gen Hosp, Dept Internal Med 2, Athens 12462, Greece

[2] Univ Athens, Sch Med, Attikon Univ Gen Hosp, Res Inst, Athens 12462, Greece

[3] Univ Athens, Dept Biochem & Mol Biol, Athens 12462, Greece

[4] Natl Hellen Res Fdn, Inst Biol Res & Biotechnol, Lab Signal Mediated Gene Express, Athens, Greece

来源：

LEUKEMIA RESEARCH | 2013年 / 37卷 / 03期

关键词：

MDS; MicroRNAs; Let-7a; Mir-17-5p; Mir-20a; ACUTE MYELOID-LEUKEMIA; E2F-DEPENDENT APOPTOSIS; TRANSCRIPTION FACTOR; GENE-EXPRESSION; E2F1; CANCER; RAS; ONCOGENE; SURVIVAL; GROWTH;

D O I：

10.1016/j.leukres.2012.11.011

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Mir-17-5p and mir-20a, members of the mir-17-92 family, down-regulate E2F1, which is over-expressed in myelodysplastic syndromes (MDS). Moreover, let-7a down-regulates KRAS, which is aberrantly expressed in MDS. We evaluated the expression of the aforementioned microRNAs in CD34+ cells of 43 MDS patients using real-time PCR and their target proteins (E2F1, MYC, BCL2, CCND1, and KRAS) by Western blot. Mir-17-5p and mir-20a were under expressed in high risk MDS patients, compared to low risk MDS patients. Similarly, let-7a was under expressed in patients with intermediate or high-risk karyotype. Interestingly, there was an inverse correlation between microRNA and the expression levels of their targets. Importantly, mir-17-5p and mir-20a constitute favorable prognostic factors in MDS, since their expression was associated with increased overall survival of MDS patients. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：251 / 258

页数：8

共 49 条

[1] let-7 microRNA functions as a potential growth suppressor in human colon cancer cells [J].