Mapping stripe rust resistance genes by BSR-Seq: YrMM58 and YrHY1 on chromosome 2AS in Chinese wheat lines Mengmai 58 and Huaiyang 1 are Yr17

被引：26

作者：

Wang, Yong ^{[1
]}

Zhang, Huaizhi ^{[1
]}

Xie, Jingzhong ^{[2
]}

Guo, Bingmin ^{[1
]}

Chen, Yongxing ^{[2
]}

Zhang, Huaiyu ^{[3
]}

Lu, Ping ^{[2
]}

Wu, Qiuhong ^{[2
]}

Li, Miaomiao ^{[1
]}

Zhang, Deyun ^{[1
]}

Guo, Guanghao ^{[1
]}

Yang, Jian ^{[2
]}

Zhang, Panpan ^{[2
]}

Zhang, Yan ^{[4
]}

Wang, Xicheng ^{[5
]}

Zhao, Hong ^{[5
]}

Cao, Tingjie ^{[5
]}

Liu, Zhiyong ^{[2
]}

机构：

[1] China Agr Univ, Coll Agron & Biotechnol, Beijing 100193, Peoples R China

[2] Chinese Acad Sci, Inst Genet & Dev Biol, State Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

[3] Sichuan Agr Univ, Coll Life Sci, Yaan 625014, Sichuan, Peoples R China

[4] China Agr Univ, Coll Hort, Beijing 100193, Peoples R China

[5] Henan Acad Agr Sci, Wheat Res Inst, Zhengzhou 450002, Henan, Peoples R China

来源：

CROP JOURNAL | 2018年 / 6卷 / 01期

关键词：

Genetic mapping; Stripe rust; Triticum aestivum; BSR-seq; SNP; BULKED SEGREGANT ANALYSIS; F-SP TRITICI; PUCCINIA-STRIIFORMIS; AEGILOPS-TAUSCHII; IDENTIFICATION; GENOME; SEQUENCE; TEMPERATURE; EVOLUTION; PATHOGENS;

D O I：

10.1016/j.cj.2017.03.002

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Stripe rust (yellow rust), caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating fungal diseases in common wheat (Triticum aestivum L.) in China and worldwide. Resistance breeding is the most effective strategy to control diseases in crop plants. Chinese wheat lines Mengmai 58 and Huaiyang 1 are highly resistant to PST race CYR34 (V26) at the adult plant stage. To genetically map the underlying resistance genes we developed segregating populations by crossing Mengmai 58 and Huaiyang 1 with the susceptible cultivar Nongda 399. The stripe rust resistances in Mengmai 58 and Huaiyang 1 were both controlled by single dominant genes, provisionally designated YrMM58 and YrHY1, respectively. Bulked segregant RNA-Seq (BSR-Seq) analysis showed that YrMM58 and YrHY1 were located in the same distal similar to 16 Mb region on chromosome 2AS. Comparative genomics analysis with the physical map of Aegilops tauschii proved useful for developing additional markers to saturate the genetic linkage map. YrMM58 and YrHY1 were mapped to the distal end of chromosome arm 2AS, with the closest marker WGGB148 being 7.7 cM and 3.8 cM from the resistance gene, which was considered to be Yr17. These markers can be used in marker-assisted selection. (C) 2017 "Crop Science Society of China and Institute of Crop Science, CAAS". Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

引用

页码：91 / 98

页数：8

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