Two dimensional α-MoO3-x nanoflakes as bare eye probe for hydrogen peroxide in biological fluids

被引：27

作者：

Sharma, Amit Kumar ^{[1
]}

Pandey, Sunil ^{[1
]}

Sharma, Krishna Hari ^{[6
]}

Nerthigan, Yowan ^{[1
]}

Khan, M. Shahnawaz ^{[1
,3
,4
]}

Hang, Da-Ren ^{[6
]}

Wu, Hui-Fen ^{[1
,2
,3
,4
,5
]}

机构：

[1] Natl Sun Yat Sen Univ, Dept Chem, 70 Lien Hai Rd, Kaohsiung 80424, Taiwan

[2] Kaohsiung Med Univ, Sch Pharm, Coll Pharm, Kaohsiung 807, Taiwan

[3] Natl Sun Yat Sen Univ, Doctoral Degree Program Marine Biotechnol, Kaohsiung 80424, Taiwan

[4] Acad Sinica, Kaohsiung 80424, Taiwan

[5] Natl Sun Yat Sen Univ, Inst Med Sci & Technol, Kaohsiung 80424, Taiwan

[6] Natl Sun Yat Sen Univ, Dept Mat & Optoelect Sci, Coll Engn, Kaohsiung 80424, Taiwan

来源：

ANALYTICA CHIMICA ACTA | 2018年 / 1015卷

关键词：

Two-dimensional nanomaterial; Bare eye; Rapid detection; H2O2; Colorimetric assay; Urine bioassay; MOLYBDENUM OXIDE; VISUAL DETECTION; COLORIMETRIC DETECTION; GOLD NANOPARTICLES; PLASMON RESONANCES; CATALYTIC-ACTIVITY; OXYGEN VACANCIES; QUANTUM DOTS; MOO3; GLUCOSE;

D O I：

10.1016/j.aca.2018.01.057

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We report a rapid and facile method for detection of hydrogen peroxide (H2O2) in biological fluids using sub-stoichiometric two-dimensional (2D) molybdenum trioxide (alpha-MoO3-x) nanoflakes. The two-dimensional nanoflakes, initially blue in color, is oxidized after interaction with hydrogen peroxide thereby changing its oxidation state to form alpha-MoO3. The change in oxidation state of nanoflakes transforms from blue to a visually distinct hazy blue color with change in absorption spectrum. The phenomenal property is explored here in sensing up to 34 nM as limit of detection. The efficacy of the detection system was analyzed by "zone of inhibition" based agar diffusion assay with different concentrations of H2O2. The current approach is highly accurate, effective and reproducible for quantification of physiological concentration of H2O2 in biological fluid such as human urine. (C) 2018 Elsevier B.V. All rights reserved.

引用

页码：58 / 65

页数：8