Automated cell counts on CSF samples: A multicenter performance evaluation of the GloCyte system

被引：7

作者：

Hod, E. A. ^{[1
,2
]}

Brugnara, C. ^{[3
,4
]}

Pilichowska, M. ^{[5
]}

Sandhaus, L. M. ^{[6
]}

Luu, H. S. ^{[7
]}

Forest, S. K. ^{[1
,2
]}

Netterwald, J. C. ^{[1
]}

Reynafarje, G. M. ^{[2
]}

Kratz, A. ^{[1
,2
]}

机构：

[1] Columbia Univ Coll Phys & Surg, Dept Pathol & Cell Biol, 630 W 168th St, New York, NY 10032 USA

[2] NewYork Presbyterian Hosp, New York, NY USA

[3] Boston Childrens Hosp, Dept Lab Med, Boston, MA USA

[4] Harvard Med Sch, Boston, MA USA

[5] Tufts Med Ctr, Boston, MA USA

[6] Univ Hosp, Cleveland Med Ctr, Cleveland, OH USA

[7] Childrens Med Ctr Dallas, Dallas, TX USA

来源：

INTERNATIONAL JOURNAL OF LABORATORY HEMATOLOGY | 2018年 / 40卷 / 01期

关键词：

automated cell counters; cerebrospinal fluid testing; laboratory testing; red cell counts; white cell counts; BODY-FLUID MODE; REFLEX TESTING RULES; CEREBROSPINAL-FLUID; HEMATOLOGY ANALYZERS; SYSMEX XN-1000; ASCITIC FLUIDS; BLOOD-CELLS; XE-5000;

D O I：

10.1111/ijlh.12728

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objectives: Automated cell counters have replaced manual enumeration of cells in blood and most body fluids. However, due to the unreliability of automated methods at very low cell counts, most laboratories continue to perform labor-intensive manual counts on many or all cerebrospinal fluid (CSF) samples. This multicenter clinical trial investigated if the GloCyte System (Advanced Instruments, Norwood, MA), a recently FDA-approved automated cell counter, which concentrates and enumerates red blood cells (RBCs) and total nucleated cells (TNCs), is sufficiently accurate and precise at very low cell counts to replace all manual CSF counts. Methods: The GloCyte System concentrates CSF and stains RBCs with fluorochrome-labeled antibodies and TNCs with nucleic acid dyes. RBCs and TNCs are then counted by digital image analysis. Residual adult and pediatric CSF samples obtained for clinical analysis at five different medical centers were used for the study. Cell counts were performed by the manual hemocytometer method and with the GloCyte System following the same protocol at all sites. The limits of the blank, detection, and quantitation, as well as precision and accuracy of the GloCyte, were determined. Results: The GloCyte detected as few as 1 TNC/mu L and 1 RBC/mu L, and reliably counted as low as 3 TNCs/mu L and 2RBCs/L. The total coefficient of variation was less than 20%. Comparison with cell counts obtained with a hemocytometer showed good correlation (>97%) between the GloCyte and the hemocytometer, including at very low cell counts. Conclusions: The GloCyte instrument is a precise, accurate, and stable system to obtain red cell and nucleated cell counts in CSF samples. It allows for the automated enumeration of even very low cell numbers, which is crucial for CSF analysis. These results suggest that GloCyte is an acceptable alternative to the manual method for all CSF samples, including those with normal cell counts.

引用

页码：56 / 65

页数：10