An engineered split M.Hhal-zinc finger fusion lacks the intended methyltransferase specificity

被引：10

作者：

Meister, Glenna E. ^{[1
]}

Chandrasegaran, Srinivasan ^{[2
]}

Ostermeier, Marc ^{[1
]}

机构：

[1] Johns Hopkins Univ, Dept Chem & Biomol Engn, Baltimore, MD 21218 USA

[2] Johns Hopkins Univ, Bloomberg Sch Publ Hlth, Dept Environm Hlth Sci, Baltimore, MD 21205 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 377卷 / 01期

关键词：

DNA methylation; Zinc fingers; Protein engineering;

D O I：

10.1016/j.bbrc.2008.09.099

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ability to site-specifically methylate DNA in vivo would have wide applicability to the study of basic biomedical problems as well as enable studies on the potential of site-specific DNA methylation as a therapeutic strategy for the treatment of diseases. Natural DNA methyltransferases lack the specificity required for these applications. Nomura and Barbas [W. Nomura, C.F. Barbas 3rd, In vivo site-specific DNA methylation with a designed sequence-enabled DNA methylase, J. Am. Chem. Soc. 129 (2007) 8676-8677] have reported that an engineered DNA methyltransferase comprised of fragments of M.Hhal methyltransferase and zinc finger proteins has very high specificity for the chosen target site. Our analysis of this engineered enzyme shows that the fusion protein methylates target and non-target sites with similar efficiency. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：226 / 230

页数：5

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